Characterization of Two Novel Linear B-Cell Epitopes in the Capsid Protein of Avian Hepatitis E Virus (HEV) That Are Common to Avian, Swine, and Human HEVs

Journal of Virology
Xinjie WangEn-Min Zhou

Abstract

Antisera raised against the avian hepatitis E virus (HEV) capsid protein are cross-reactive with human and swine HEV capsid proteins. In this study, two monoclonal antibodies (MAbs) against the avian HEV capsid protein, namely, 3E8 and 1B5, were shown to cross-react with the swine HEV capsid protein. The motifs involved in binding both MAbs were identified and characterized using phage display biopanning, peptide synthesis, and truncated or mutated protein expression, along with indirect enzyme-linked immunosorbent assay (ELISA) and Western blotting. The results showed that the I/VPHD motif is a necessary core sequence and that P and H are two key amino acids for recognition by MAb 3E8. The VKLYM/TS motif is the minimal amino acid sequence necessary for recognition by MAb 1B5. Cross-reactivity between the two epitopes and antibodies against avian, swine, and human HEVs in sera showed that both epitopes are common to avian, swine, and human HEVs. In addition, amino acid sequence alignment of the capsid proteins revealed that the key motifs of both novel epitopes are the same in HEVs from different animal species, predicting that they may be common to HEV isolates from boars, rabbits, rats, ferrets, mongooses, deer, and camels as...Continue Reading

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Citations

Jan 21, 2016·Journal of Biomedical Science·Chien-Hsun WuHan-Chung Wu
Sep 6, 2015·Veterinary Microbiology·Qin ZhaoEn-Min Zhou
Dec 20, 2017·Frontiers in Microbiology·Yuchen NanEn-Min Zhou
Aug 10, 2019·Frontiers in Microbiology·Peng SunQin Zhao
Jun 16, 2021·Applied Microbiology and Biotechnology·Barbara KubickovaRainer G Ulrich
Oct 12, 2021·Applied Microbiology and Biotechnology·Beibei ZhangEn-Min Zhou

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