Characterization of two pigeon paramyxovirus type 1 isolates in China

Avian Pathology : Journal of the W.V.P.A
Abie AwuGuo-zhong Zhang

Abstract

For over three decades, there has been a continuing panzootic caused by a virulent variant avian paramyxovirus type 1 strain, the so-called pigeon paramyxovirus type 1. It is found primarily in racing pigeons, but it has also spread to wild birds and poultry. In this study, two pigeon paramyxovirus type 1 strains, SD12 and BJ13, obtained from diseased pigeons in China, were characterized. Phylogenetic analysis based on complete sequences allowed characterization of both strains as genotype VI, class II. Further phylogenetic analysis of a 374-nucleotide section of the fusion gene showed that SD12 fell into lineage VIbii-d and BJ13 into VIbii-f. The deduced amino acid sequence of the cleavage site of the fusion protein confirmed that both isolates contained the virulent motif (112)K/RRQKR↓F(117) at the cleavage site. Nevertheless, the values of intracerebral pathogenicity indices showed the SD12 isolate to be a velogenic strain and BJ13 isolate to be a mesogenic strain. The SD12 isolate was further investigated via clinical observation, RNA detection, histopathology and viral serology in experimentally infected 3-week-old chickens. It showed a mild pathological phenotype in chickens, with viral replication restricted to a few tis...Continue Reading

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Datasets Mentioned

BETA
KJ808820
GQ338311
JX486552
JX901124
AF077761

Methods Mentioned

BETA
PCRs
electrophoresis
PCR
dissection
light microscopy

Software Mentioned

ClustalW
MEGA4
MegAlign
DNAstar

Related Concepts