Characterization of two putative protein phosphatase genes and their involvement in phosphorus efficiency in Phaseolus vulgaris

Journal of Integrative Plant Biology
Cuiyue LiangHong Liao

Abstract

Protein dephosphorylation mediated by protein phosphatases plays a major role in signal transduction of plant responses to environmental stresses. In this study, two putative protein phosphatases, PvPS2:1 and PvPS2:2 were identified and characterized in bean (Phaseolus vulgaris). The two PvPS2 members were found to be localized to the plasma membrane and the nucleus by transient expression of PvPS2:GFP in onion epidermal cells. Transcripts of the two PvPS2 genes were significantly increased by phosphate (P(i) ) starvation in the two bean genotypes, G19833 (a P-efficient genotype) and DOR364 (a P-inefficient genotype). However, G19833 exhibited higher PvPS2:1 expression levels than DOR364 in both leaves and roots during P(i) starvation. Increased transcription of PvPS2:1 in response to P(i) starvation was further verified through histochemical analysis of PvPS2:1 promoter fusion ß-glucuronidase (GUS) in transgenic Arabidopsis plants. Analysis of PvPS2:1 overexpression lines in bean hairy roots and Arabidopsis showed that PvS2:1 was involved in root growth and P accumulation. Furthermore, expression levels of two P(i) starvation responsive genes were upregulated and the APase activities were enhanced in the overexpressing PvPS2:1...Continue Reading

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Citations

Mar 1, 2014·Plant Science : an International Journal of Experimental Plant Biology·Xianliang LiLiangcai Peng
May 26, 2017·Cellular & Molecular Biology Letters·Md Mahmudul HasanXuexian Li
May 14, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zhandong CaiHai Nian
Nov 20, 2020·Journal of Experimental Botany·D M S B DissanayakaWilliam C Plaxton
Nov 14, 2020·The Plant Journal : for Cell and Molecular Biology·Devang MehtaR Glen Uhrig

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