Characterization of type I and type II diacylglycerol acyltransferases from the emerging model alga Chlorella zofingiensis reveals their functional complementarity and engineering potential

Biotechnology for Biofuels
Xuemei MaoJin Liu

Abstract

The green alga Chlorella zofingiensis has been recognized as an industrially relevant strain because of its robust growth under multiple trophic conditions and the potential for simultaneous production of triacylglycerol (TAG) and the high-value keto-carotenoid astaxanthin. Nevertheless, the mechanism of TAG synthesis remains poorly understood in C. zofingiensis. Diacylglycerol acyltransferase (DGAT) is thought to catalyze the committed step of TAG assembly in the Kennedy pathway. C. zofingiensis genome is predicted to possess eleven putative DGAT-encoding genes, the greatest number ever found in green algae, pointing to the complexity of TAG assembly in the alga. The transcription start site of C. zofingiensis DGATs was determined by 5'-rapid amplification of cDNA ends (RACE), and their coding sequences were cloned and verified by sequencing, which identified ten DGAT genes (two type I DGATs designated as CzDGAT1A and CzDGAT1B, and eight type II DGATs designated as CzDGTT1 through CzDGTT8) and revealed that the previous gene models of seven DGATs were incorrect. Function complementation in the TAG-deficient yeast strain confirmed the functionality of most DGATs, with CzDGAT1A and CzDGTT5 having the highest activity. In vitro D...Continue Reading

References

Jul 1, 1995·The Plant Cell·J Ohlrogge, J Browse
Dec 14, 2001·The Journal of Biological Chemistry·Line SandagerSten Stymne
Dec 23, 2003·Applied Microbiology and Biotechnology·J A Del CampoM G Guerrero
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Brook K NelsonAndreas Nebenführ
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Qiang HuAl Darzins
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Rainer WaadtJörg Kudla
Sep 17, 2008·Applied Microbiology and Biotechnology·Yanqun LiChristopher Q Lan
Jul 10, 2010·Bioresource Technology·Jin LiuFeng Chen
Nov 18, 2011·The Journal of Biological Chemistry·Kent D Chapman, John B Ohlrogge
Jan 1, 2010·The Arabidopsis Book·Yonghua Li-BeissonJohn Ohlrogge
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 15, 2014·PLoS Genetics·Dongmei WangJian Xu
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Fengjuan LiHanhua Hu
May 30, 2015·Journal of Experimental Botany·Mahmoud GargouriDavid R Gang
Dec 4, 2015·Biotechnology for Biofuels·Adrián López García de LomanaNitin S Baliga
Feb 18, 2016·Biochimica Et Biophysica Acta·Krzysztof ZienkiewiczChristoph Benning

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Citations

Mar 5, 2019·The Plant Journal : for Cell and Molecular Biology·Jin LiuWenqiang Yang
Jan 29, 2020·The Plant Journal : for Cell and Molecular Biology·Yang XuGuanqun Chen
Jun 17, 2020·The Plant Journal : for Cell and Molecular Biology·Lucas J FalarzGuanqun Chen

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Methods Mentioned

BETA
acylation
one-hybrid
transgenic
PCR
electrophoresis

Software Mentioned

ClustalX
Image J
PredAlgo
SPSS
ClustalX2
MEGA6
TargetP
PlantPAN
JBrowse
DGAT

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