Abstract
We have investigated the genomic organization of Entamoeba histolytica ubiquitin and looked for the occurrence of a ubiquitin-conjugating system in this organism. Southern blots indicated the presence of > or = 5 ubiquitin-coding regions. One of these, EhUBI1, was cloned and sequenced and found to correspond to a monoubiquitin gene; as shown by a polymerase chain reaction, E. histolytica lacked polyubiquitin genes altogether. Blots of poly(A)+ RNA from exponentially-growing trophozoite cultures exhibited five ubiquitin transcripts, the most prominent and smallest of which corresponded to EhUBI1 mRNA. Expression of the ubiquitin genes was not influenced by heat shock. Although the predicted amino acid sequence of the ubiquitin from E. histolytica differs significantly (in 7-9 amino acid residues) from that of yeast and animals, expression of the coding sequence of EhUBI1 suppressed the heat-sensitive phenotype of a polyubiquitin gene-deficient yeast mutant. In correlation, trophozoite extract catalyzed an ATP-dependent conjugation of radioiodinated bovine ubiquitin to trophozoite proteins. The latter data indicate that E. histolytica contains a functional ubiquitin-conjugating system.
References
Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S DiamondC C Cunnick
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 27, 1979·Biochemistry·J M ChirgwinW J Rutter
Aug 10, 1992·FEBS Letters·C WöstmannT Bakker-Grunwald
Jan 1, 1992·Annual Review of Genetics·S Jentsch
Aug 1, 1992·Molecular and Biochemical Parasitology·E TannichR D Horstmann
Jun 13, 1991·Biochimica Et Biophysica Acta·S JentschH P Hauser
Jan 1, 1991·Annual Review of Cell Biology·D Finley, V Chau
Nov 1, 1991·Molecular and Biochemical Parasitology·E TannichR D Horstmann
Jan 29, 1988·Science·R K SaikiH A Erlich
Mar 14, 1988·FEBS Letters·A Müller-TaubenbergerG Gerisch
Jan 6, 1989·Science·M L SoginD A Peattie
Mar 27, 1987·Cell·D FinleyA Varshavsky
Dec 30, 1988·Gene·R D Gietz, A Sugino
Nov 1, 1995·Journal of Molecular Evolution·P J Keeling, W F Doolittle
Jan 1, 1994·Methods in Enzymology·H Scholze, E Tannich
Oct 7, 1994·Cell·A Ciechanover
Jul 1, 1994·The Journal of Eukaryotic Microbiology·R Manning-CelaI Meza
May 2, 1994·FEBS Letters·H KrebberT Bakker-Grunwald
Nov 1, 1993·The American Journal of Tropical Medicine and Hygiene·D J LowrieR M Ventullo
Dec 1, 1993·DNA and Cell Biology·I BruchhausE Tannich
Jul 12, 1993·FEBS Letters·T Wenzel, W Baumeister
Mar 15, 1996·The Journal of Biological Chemistry·H ScholzeT Bakker-Grunwald
Jan 1, 1996·Trends in Biochemical Sciences·S HayashiS Matsufuji
Citations
Jan 17, 2012·Parasitology Research·Shweta AryaSwati Tiwari
Jul 15, 2003·Experimental Parasitology·Ricardo SánchezRoberto P Stock
May 26, 1999·Molecular and Biochemical Parasitology·R SharmaA Bhattacharya
Oct 8, 1999·Molecular and Biochemical Parasitology·C D Robertson
Sep 22, 1999·European Journal of Biochemistry·J GonzalezD Eichinger
Dec 5, 2012·The Journal of Biological Chemistry·Dustin E Bosch, David P Siderovski
Oct 2, 2007·Eukaryotic Cell·Elizabeth L Ponder, Matthew Bogyo
Jan 5, 2011·Future Microbiology·Ayala Tovy, Serge Ankri
Nov 15, 2012·Experimental Parasitology·Yan HuangXinbing Yu
Oct 11, 2008·Experimental Parasitology·Margit TazreiterMichael Duchêne
Jan 30, 2014·Cellular Microbiology·Sophia KatzSerge Ankri
Aug 10, 2019·Cells·Sara Martín-VillanuevaJesús de la Cruz
May 1, 2021·International Journal of Molecular Sciences·Sara Martín-VillanuevaJesús de la Cruz