PMID: 8943152Nov 12, 1996Paper

Characterization of ubiquitin genes and -transcripts and demonstration of a ubiquitin-conjugating system in Entamoeba histolytica

Molecular and Biochemical Parasitology
C WöstmannT Bakker-Grunwald

Abstract

We have investigated the genomic organization of Entamoeba histolytica ubiquitin and looked for the occurrence of a ubiquitin-conjugating system in this organism. Southern blots indicated the presence of > or = 5 ubiquitin-coding regions. One of these, EhUBI1, was cloned and sequenced and found to correspond to a monoubiquitin gene; as shown by a polymerase chain reaction, E. histolytica lacked polyubiquitin genes altogether. Blots of poly(A)+ RNA from exponentially-growing trophozoite cultures exhibited five ubiquitin transcripts, the most prominent and smallest of which corresponded to EhUBI1 mRNA. Expression of the ubiquitin genes was not influenced by heat shock. Although the predicted amino acid sequence of the ubiquitin from E. histolytica differs significantly (in 7-9 amino acid residues) from that of yeast and animals, expression of the coding sequence of EhUBI1 suppressed the heat-sensitive phenotype of a polyubiquitin gene-deficient yeast mutant. In correlation, trophozoite extract catalyzed an ATP-dependent conjugation of radioiodinated bovine ubiquitin to trophozoite proteins. The latter data indicate that E. histolytica contains a functional ubiquitin-conjugating system.

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Citations

Jul 15, 2003·Experimental Parasitology·Ricardo SánchezRoberto P Stock
May 26, 1999·Molecular and Biochemical Parasitology·R SharmaA Bhattacharya
Oct 8, 1999·Molecular and Biochemical Parasitology·C D Robertson
Dec 5, 2012·The Journal of Biological Chemistry·Dustin E Bosch, David P Siderovski
Jan 5, 2011·Future Microbiology·Ayala Tovy, Serge Ankri
Nov 15, 2012·Experimental Parasitology·Yan HuangXinbing Yu
May 1, 2021·International Journal of Molecular Sciences·Sara Martín-VillanuevaJesús de la Cruz

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