Characterization of uncultivable bat influenza virus using a replicative synthetic virus

PLoS Pathogens
Bin ZhouWenjun Ma

Abstract

Bats harbor many viruses, which are periodically transmitted to humans resulting in outbreaks of disease (e.g., Ebola, SARS-CoV). Recently, influenza virus-like sequences were identified in bats; however, the viruses could not be cultured. This discovery aroused great interest in understanding the evolutionary history and pandemic potential of bat-influenza. Using synthetic genomics, we were unable to rescue the wild type bat virus, but could rescue a modified bat-influenza virus that had the HA and NA coding regions replaced with those of A/PR/8/1934 (H1N1). This modified bat-influenza virus replicated efficiently in vitro and in mice, resulting in severe disease. Additional studies using a bat-influenza virus that had the HA and NA of A/swine/Texas/4199-2/1998 (H3N2) showed that the PR8 HA and NA contributed to the pathogenicity in mice. Unlike other influenza viruses, engineering truncations hypothesized to reduce interferon antagonism into the NS1 protein didn't attenuate bat-influenza. In contrast, substitution of a putative virulence mutation from the bat-influenza PB2 significantly attenuated the virus in mice and introduction of a putative virulence mutation increased its pathogenicity. Mini-genome replication studies a...Continue Reading

References

Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·J TalonP Palese
Apr 16, 2005·Science·Kartik ChandranJames M Cunningham
Jun 16, 2005·Journal of Virology·Michelle QuinlivanPeter Palese
Dec 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·G GabrielJ Stech
Jul 19, 2006·Clinical Microbiology Reviews·Charles H CalisherTony Schountz
Sep 18, 2008·The Journal of General Virology·Benjamin G HaleDavid Jackson
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Qinshan Gao, Peter Palese
Jan 19, 2010·Nature Structural & Molecular Biology·Daisy W LeungGaya K Amarasinghe
Jan 20, 2010·PLoS Pathogens·Charalampos ValmasElke Mühlberger
Oct 12, 2010·Vaccine·Bin ZhouDavid E Wentworth
May 18, 2011·Nucleic Acids Research·Kensuke NakamuraShigehiko Kanaya
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Suxiang TongRuben O Donis
Apr 25, 2012·Methods in Molecular Biology·Bin Zhou, David E Wentworth
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qing LiGeorge F Gao
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xueyong ZhuIan A Wilson
Oct 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Adolfo García-Sastre
Nov 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Parameshwaran RamananGaya K Amarasinghe
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xueyong ZhuIan A Wilson
May 17, 2013·Science Translational Medicine·Philip R DormitzerJ Craig Venter
May 29, 2013·Influenza and Other Respiratory Viruses·Daniel DlugolenskiRalph A Tripp
Oct 17, 2013·PLoS Pathogens·Suxiang TongRuben O Donis
Feb 7, 2014·Journal of Clinical Microbiology·Bin ZhouDavid E Wentworth
Mar 4, 2014·Trends in Microbiology·Ying WuGeorge F Gao

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Citations

Nov 26, 2015·Virology·Junki MaruyamaAyato Takada
Mar 8, 2016·Current Opinion in Virology·Linda BrunotteMartin Schwemmle
Mar 18, 2016·Emerging Infectious Diseases·Hiroshi KatohMinoru Kidokoro
May 13, 2015·PloS One·Gudrun Stephanie FreidlMarion Koopmans
Sep 22, 2016·Nature Communications·Étori Aguiar MoreiraMindaugas Juozapaitis
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Étori Aguiar MoreiraGert Zimmer
Dec 15, 2017·Journal of Virology·Hannah L TurkingtonBenjamin G Hale
Aug 8, 2018·Veterinary Sciences·Suresh V Kuchipudi, Ruth H Nissly
Aug 8, 2015·Journal of Virology·Hannah L TurkingtonBenjamin G Hale
Jul 22, 2016·Journal of Virology·Bin ZhouDavid E Wentworth
May 14, 2019·The Journal of General Virology·Guanlong XuJiabo Ding
Sep 15, 2017·The Journal of General Virology·Kevin CiminskiMartin Schwemmle
Feb 6, 2019·Scientific Reports·Masahiro SatoAyato Takada
Jun 26, 2020·Frontiers in Cellular and Infection Microbiology·Efstathios S Giotis
Dec 2, 2020·The Science of the Total Environment·Ghulam NabiDongming Li
Dec 25, 2019·Cold Spring Harbor Perspectives in Medicine·Kevin Ciminski, Martin Schwemmle
Apr 4, 2021·Viruses·Wenyu YangWenjun Ma

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Methods Mentioned

BETA
transfection
following
Assay

Related Concepts