Characterization of urea transport mechanisms in the intestinal tract of growing pigs

American Journal of Physiology. Gastrointestinal and Liver Physiology
Jack E C KroneDaniel A Columbus

Abstract

Pigs are capable of nitrogen salvage via urea recycling, which involves the movement of urea in the gastrointestinal tract. Aquaporins (AQP) and urea transporter B (UT-B) are involved in urea recycling in ruminants; however, their contribution to urea flux in the intestinal tract of the pig is not known. The objective of this study was to characterize the presence and relative contribution of known urea transporters to urea flux in the growing pig. Intestinal tissue samples (duodenum, jejunum, ileum, cecum, and colon) were obtained from nine barrows (50.8 ± 0.9 kg) and analyzed for mRNA abundance of UT-B and AQP-3, -7, and -10. Immediately after tissue collection, samples from the jejunum and cecum were placed in Ussing chambers for analysis of the serosal-to-mucosal urea flux (Jsm-urea) with no inhibition or when incubated in the presence of phloretin to inhibit UT-B-mediated transport, NiCl2 to inhibit AQP-mediated transport, or both inhibitors. UT-B expression was greatest (P < 0.05) in the cecum, whereas AQP-3, -7, and -10 expression was greatest (P < 0.05) in the jejunum. The Jsm-urea was greater in the cecum than the jejunum (67.8 . 42.7 ± 5.01 µmol·cm-2·h-1; P < 0.05), confirming the capacity for urea recycling in the gu...Continue Reading

References

Feb 1, 1986·Archiv für Tierernährung·S Buraczewski
Mar 1, 1973·Gut·A VinceF O'Grady
Jan 1, 1980·Journal of Nutritional Science and Vitaminology·N TanakaH Yoshimura
Jul 1, 1995·The Proceedings of the Nutrition Society·A A Jackson
Mar 1, 1997·The American Journal of Physiology·H YounesC Demigné
Aug 26, 1998·Annual Review of Nutrition·M F Fuller, P J Reeds
May 20, 1999·The Journal of Physiology·T Ma, A S Verkman
Jun 27, 2000·The Journal of Nutrition·C C Metges
Feb 6, 2003·American Journal of Physiology. Endocrinology and Metabolism·David TorrallardonaMalcolm F Fuller
Mar 20, 2003·Journal of Animal Science·J C Marini, M E Van Amburgh
Apr 4, 2003·The Journal of Nutrition·David TorrallardonaMalcolm F Fuller
Mar 1, 1960·Journal of Clinical Pathology·J K FAWCETT, J E SCOTT
Feb 27, 2004·American Journal of Physiology. Cell Physiology·Hideki InoueSerena M Bagnasco
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Hongyu LiMartin J Hug
Nov 26, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·N LucienM M Trinh-Trang-Tan
Mar 15, 2005·International Journal of Food Sciences and Nutrition·Alan A JacksonStephen A Wootton
Mar 31, 2005·American Journal of Physiology. Renal Physiology·Hideki InoueSerena M Bagnasco
Apr 23, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·G S StewartC P Smith
Jun 1, 2005·Pflügers Archiv : European journal of physiology·Serena M Bagnasco
Aug 1, 1995·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·J M HibbertC Persaud
Aug 19, 2007·BMC Molecular Biology·Ann-Britt NygardMerete Fredholm
Oct 27, 2007·Annual Review of Physiology·Aleksandra RojekRobert A Fenton
Jun 1, 2005·Nutrition Research Reviews·Gavin S Stewart, Craig P Smith
Feb 11, 2009·Journal of Agricultural and Food Chemistry·Warren Miner-WilliamsMalcolm F Fuller
May 1, 2009·The Journal of Nutrition·Aileen Joy O Libao-MercadoCornelis F M de Lange
Nov 21, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·D CollinsG S Stewart
Nov 21, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Khalid AbdounHolger Martens
Jan 5, 2010·Developmental and Comparative Immunology·Timothée BruelFrançois Meurens
Jun 29, 2010·Journal of Animal Science·A S MuscherK Huber
Dec 24, 2011·Genetics and Molecular Research : GMR·V MéndezD González
Jul 26, 2012·Journal of Animal Science·A S Muscher-BanseG Breves
Jul 3, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·F StumpffJ R Aschenbach
Aug 1, 2015·Animal : an International Journal of Animal Bioscience·D A ColumbusC F M de Lange
Jul 30, 2016·International Journal of Molecular Sciences·Alessandra PelagalliRosaria Meli
Mar 1, 2017·Journal of Agricultural and Food Chemistry·Liuqin HeYulong Yin

❮ Previous
Next ❯

Citations

Jun 23, 2020·Mediators of Inflammation·Jing GaoYuLong Yin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The American Journal of Physiology
J M SandsR B Gunn
Annual Review of Physiology
J M Sands
American Journal of Physiology. Renal Physiology
Serena M Bagnasco
© 2021 Meta ULC. All rights reserved