Characterization of vascular endothelial progenitor cells from chicken bone marrow.

BMC Veterinary Research
Chunyu BaiYuehui Ma

Abstract

Endothelial progenitor cells (EPC) are a type of stem cell used in the treatment of atherosclerosis, vascular injury and regeneration. At present, most of the EPCs studied are from human and mouse, whereas the study of poultry-derived EPCs has rarely been reported. In the present study, chicken bone marrow-derived EPCs were isolated and studied at the cellular level using immunofluorescence and RT-PCR. We found that the majority of chicken EPCs were spindle shaped. The growth-curves of chicken EPCs at passages (P) 1, -5 and -9 were typically "S"-shaped. The viability of chicken EPCs, before and after cryopreservation was 92.2% and 81.1%, respectively. Thus, cryopreservation had no obvious effects on the viability of chicken EPCs. Dil-ac-LDL and FITC-UAE-1 uptake assays and immunofluorescent detection of the cell surface markers CD34, CD133, VEGFR-2 confirmed that the cells obtained in vitro were EPCs. Observation of endothelial-specific Weibel-Palade bodies using transmission electron microscopy further confirmed that the cells were of endothelial lineage. In addition, chicken EPCs differentiated into endothelial cells and smooth muscle cells upon induction with VEGF and PDGF-BB, respectively, suggesting that the chicken EPCs r...Continue Reading

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Citations

Oct 29, 2013·Experimental Cell Research·Yan-Qing HeXuesong Yang
Mar 27, 2015·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Yi-Sen ChengXue-Song Yang
Jan 30, 2015·American Journal of Veterinary Research·Margaret M SalterAnne A Wooldridge
Oct 21, 2018·BMC Microbiology·Raissa L DavisMathilde Richard

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Methods Mentioned

BETA
transmission electron microscopy
electrophoresis

Software Mentioned

JMP® Discovery
Gelpro32

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