Characterization of Vibrio parahaemolyticus clinical strains from Maryland (2012-2013) and comparisons to a locally and globally diverse V. parahaemolyticus strains by whole-genome sequence analysis

Frontiers in Microbiology
Julie HaendigesNarjol González-Escalona

Abstract

Vibrio parahaemolyticus is the leading cause of foodborne illnesses in the US associated with the consumption of raw shellfish. Previous population studies of V. parahaemolyticus have used Multi-Locus Sequence Typing (MLST) or Pulsed Field Gel Electrophoresis (PFGE). Whole genome sequencing (WGS) provides a much higher level of resolution, but has been used to characterize only a few United States (US) clinical isolates. Here we report the WGS characterization of 34 genomes of V. parahaemolyticus strains that were isolated from clinical cases in the state of Maryland (MD) during 2 years (2012-2013). These 2 years saw an increase of V. parahaemolyticus cases compared to previous years. Among these MD isolates, 28% were negative for tdh and trh, 8% were tdh positive only, 11% were trh positive only, and 53% contained both genes. We compared this set of V. parahaemolyticus genomes to those of a collection of 17 archival strains from the US (10 previously sequenced strains and 7 from NCBI, collected between 1988 and 2004) and 15 international strains, isolated from geographically-diverse environmental and clinical sources (collected between 1980 and 2010). A WGS phylogenetic analysis of these strains revealed the regional outbreak ...Continue Reading

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Citations

Jun 18, 2016·Emerging Infectious Diseases·Narjol Gonzalez-EscalonaJaime Martinez-Urtaza
May 18, 2017·Foodborne Pathogens and Disease·Mohammad Shamsur RahmanLuca Fasolato
Dec 16, 2016·Journal of Clinical Microbiology·Feng XuCheryl A Whistler
Mar 20, 2016·Applied and Environmental Microbiology·Julie HaendigesNarjol Gonzalez-Escalona
Mar 24, 2017·Journal of Clinical Microbiology·Narjol Gonzalez-EscalonaJaime Martinez-Urtaza
Jan 19, 2018·Journal of Applied Microbiology·P A RodriguesC A Conte-Junior
Sep 30, 2018·Environmental Science and Pollution Research International·Abolade A OyeladeObasola Ezekiel Fagade
Jun 3, 2017·Frontiers in Cellular and Infection Microbiology·Dongsheng HanChongxu Han
Nov 16, 2016·Trends in Microbiology·Craig Baker-AustinJaime Martinez-Urtaza

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Methods Mentioned

BETA
Electrophoresis
genotyping

Software Mentioned

CLC Genomics Workbench (
CLC Genomics workbench
kSNP
goeBURST
BIGSdb genome comparator
CLC Genome Workbench
MLST
eBURST

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