Abstract
Commercial concentrates of factor VIII (FVIII) were analyzed in order to 1) determine the effects of viral inactivation on von Willebrand factor (vWF); 2) evaluate the vWF content of the new, immunopurified concentrates; and 3) assess their potential for correcting the long bleeding time of von Willebrand disease (vWD). Included in our study were products that had been treated to inactivate viruses; older, untreated products; and the new, immunopurified concentrates. We measured von Willebrand factor antigen (vWF:Ag), ristocetin cofactor activity (RCoF), and vWF multimeric and subunit composition. A newly developed radioimmunoassay (RIA) was used to quantitate vWF:Ag. The vWF:Ag content varied from 0.083 micrograms/IU FVIII:C for Hemofil M to 32.2 micrograms/IU FVIII:C for Humate-P, whereas pooled normal human plasma (NHP) contained 6.3 micrograms/IU FVIII:C. The RCoF varied from 0.0007 to 2.09 U/IU FVIII:C, with the immunopurified concentrates having the lowest values and Humate-P the highest. The ratio of RCoF to vWF:Ag ranged from 11 to 96 U/mg, as compared to a ratio of 160 for NHP. All of the concentrates lacked the largest vWF multimers, and all had abnormal triplet patterns. Modest differences between some untreated conc...Continue Reading
References
Dec 1, 1977·British Journal of Haematology·I M Nilsson, U Hedner
Oct 1, 1978·Thrombosis Research·K M Brinkhous, M S Read
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Mar 1, 1976·The American Journal of Medicine·D Green, E V Potter
Feb 1, 1987·The Journal of Clinical Investigation·S D BerkowitzT S Zimmerman
Jun 3, 1986·Biochemistry·M W ChopekK Titani
May 1, 1987·American Journal of Hematology·P M MannucciR Castillo
Jan 1, 1987·Vox Sanguinis·M F López-FernándezJ Batlle
Jan 1, 1987·Vox Sanguinis·C MazurierM Goudemand
Jul 15, 1987·Thrombosis Research·J IngerslevS Stenbjerg
Jan 1, 1988·Transfusion·K B ThomasP Schiff
Mar 1, 1988·European Journal of Haematology·E Berntorp, I M Nilsson
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R B LeveneD C Lynch
Mar 1, 1986·The Journal of Clinical Investigation·T S ZimmermanL H Nannini
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1966·Analytical Biochemistry·C B Laurell
Feb 1, 1980·Vox Sanguinis·J P AllainJ P Soulier
Jun 29, 1981·Biochimica Et Biophysica Acta·B A PerretE A Beck
Citations
Sep 1, 1990·The Histochemical Journal·S C WatkinsH S Slayter
Feb 24, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·H J MetznerJ Friedebold
Feb 13, 2001·Transfusion·Y P Agrawal, W Dzik
Jun 1, 1990·British Journal of Haematology·A M CummingA M Burn
Aug 1, 1991·British Journal of Haematology·C GaucherC Mazurier
Mar 1, 1991·British Journal of Haematology·E M SloandW Fricke
Apr 1, 1992·British Journal of Haematology·T FaconJ Goudemand
Jan 1, 1991·Vox Sanguinis·C MazurierM Goudemand
Jan 1, 1992·Vox Sanguinis·M Burnouf-Radosevich, T Burnouf
Jan 1, 1995·Vox Sanguinis·D L Aronson, P Chang
Jun 21, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·T BurnoufM El-Ekiaby
Sep 17, 2008·Haemophilia : the Official Journal of the World Federation of Hemophilia·W Schramm
Sep 26, 2009·Transfusion Medicine·M El-EkiabyT Burnouf
Feb 15, 1991·Thrombosis Research·M JeleńskaK Breddin
Jun 15, 1992·Thrombosis Research·P BaillodR Pflugshaupt
Aug 11, 2011·Blood·Kellie R MachlusAlisa S Wolberg
Feb 28, 2006·Biologicals : Journal of the International Association of Biological Standardization·M StadlerT-E Svae
May 1, 1999·Haemophilia : the Official Journal of the World Federation of Hemophilia·E Berntorp
Jul 21, 2004·Vox Sanguinis·T BurnoufJ Goudemand
Mar 1, 1991·American Journal of Hematology·R E WeinsteinF R Rickles
Aug 15, 1998·The American Journal of the Medical Sciences·M D Phillips, A Santhouse
Oct 1, 1990·La Ricerca in Clinica E in Laboratorio·P M MannucciM Cattaneo