Characterization of water of hydration fractions in rabbit skeletal muscle with age and time of post-mortem by centrifugal dehydration force and rehydration methods

Cell Biology International
Ivan CameronGary D Fullerton

Abstract

Centrifugal dehydration force (CDF) and rehydration isotherm (RHI) methods were used to measure and characterize hydration fractions in rabbit psoas skeletal muscle. The CDF method assessed fluid flow rate from rabbit muscle and hydration capacity of the fractions. Bulk and multiple non-bulk water fractions were identified. The non-bulk water was divisible into the following fractions: two outer non-bulk fractions, a main chain proteins backbone or double water bridge fraction, and a single water bridge fraction. The total non-bulk water amounts to about 85% of the total water in the muscle. The sizes of the water fractions (in g water/g dry mass) agree with a recently proposed molecular stoichiometric hydration model (SHM) applicable to all proteins in and out of cells (Fullerton GD, Cameron IL. Water compartments in cells. Methods Enzymol, 2007; Cameron IL, Fullerton GD. Interfacial water compartments on tendon/collagen and in cells. In: Pollack GH, Chin WC, editors. Phase transitions in cells. Dordrecht, The Netherlands: Springer, 2008). Age of the rabbit significantly slowed the flow rate of the outer non-bulk water fraction by about 50%. Also, muscle of the older rabbit (26 weeks vs. 12 weeks old) had less bulk water and l...Continue Reading

References

Oct 1, 1988·Journal of Cellular Physiology·I L CameronA Miseta
Nov 1, 1988·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·I L CameronG D Fullerton
May 1, 1970·The American Journal of Anatomy·D W HendersonM H Stromer
Feb 1, 1997·Cell Biology International·I L CameronG D Fullerton
May 1, 1965·The Anatomical Record·J J CHIAKULAS, J E PAULY
Dec 20, 2005·Cell Biology International·Gary D FullertonIvan L Cameron
Dec 27, 2005·Cell Biology International·Gary D FullertonIvan Cameron
Feb 21, 2006·Cell Biology International·Gary D Fullerton, Maxwell R Amurao
Jan 30, 2007·Journal of Magnetic Resonance Imaging : JMRI·Gary D Fullerton, Andrés Rahal

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Citations

May 20, 2009·Biophysical Journal·Johannes FelsRyszard Grygorczyk
Jan 8, 2015·Pflügers Archiv : European journal of physiology·Ryszard GrygorczykSergei N Orlov
Dec 3, 2014·Bio Systems·Douglas E FriesenJack A Tuszynski

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