Characterization of wheat straw-degrading anaerobic alkali-tolerant mixed cultures from soda lake sediments by molecular and cultivation techniques

Microbial Biotechnology
Katharina PorschM Nikolausz

Abstract

Alkaline pretreatment has the potential to enhance the anaerobic digestion of lignocellulosic biomass to biogas. However, the elevated pH of the substrate may require alkalitolerant microbial communities for an effective digestion. Three mixed anaerobic lignocellulolytic cultures were enriched from sediments from two soda lakes with wheat straw as substrate under alkaline (pH 9) mesophilic (37°C) and thermophilic (55°C) conditions. The gas production of the three cultures ceased after 4 to 5 weeks, and the produced gas was composed of carbon dioxide and methane. The main liquid intermediates were acetate and propionate. The physiological behavior of the cultures was stable even after several transfers. The enrichment process was also followed by molecular fingerprinting (terminal restriction fragment length polymorphism) of the bacterial 16S rRNA gene and of the mcrA/mrtA functional gene for methanogens. The main shift in the microbial community composition occurred between the sediment samples and the first enrichment, whereas the structure was stable in the following transfers. The bacterial communities mainly consisted of Sphingobacteriales, Clostridiales and Spirochaeta, but differed at genus level. Methanothermobacter and ...Continue Reading

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Citations

Feb 16, 2016·Microbial Biotechnology·Elisa KorenblumJan Dirk van Elsas
Sep 12, 2015·Applied Microbiology and Biotechnology·João A B SousaAlfons J M Stams
May 4, 2016·Applied Microbiology and Biotechnology·Denny PoppHeike Sträuber
Nov 30, 2019·MicrobiologyOpen·Kamyar Mogodiniyai KasmaeiSabine Kleinsteuber
Nov 21, 2017·Applied Microbiology and Biotechnology·Emine Gozde OzbayramOrhan Ince
Mar 30, 2018·Frontiers in Microbiology·Babett WintscheSabine Kleinsteuber
Jul 3, 2021·International Journal of Environmental Research and Public Health·Ogechukwu Bose ChukwumaNorli Ismail

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

- VP
Chrompack
ab S olutions
equencher
R script
aestro
mega
C lass
ene M apper

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