Characterization, tissue expression, and immunohistochemical localization of MCL3, a C-type lectin produced by Perkinsus olseni-infected Manila clams (Ruditapes philippinarum)

Fish & Shellfish Immunology
Jin Young KimMoonjae Cho

Abstract

A novel C-type lectin designated Manila clam lectin 3 (MCL3), with a molecular weight of 17.380kDa, was identified among haemocyte expressed sequence tags of Perkinsus olseni-infected Manila clams. MCL3 was expressed in Escherichia coli M15 cells and purified with a Ni-NTA His-binding resin matrix. MCL3 agglutinated rabbit erythrocytes in the presence of Ca(+2). MCL3-induced agglutination was partially inhibited by GalNAc, Man, lactose, and raffinose, whereas the polysaccharides bovine mucin type II and Candida mannan completely inhibited agglutination. MCL3 was expressed in the haemocytes of Manila clams 3 days after infection with P. olseni and 1 day after infection with Vibrio tapetis. Based on real-time PCR analysis, mRNA transcripts of MCL3 were present in the adductor, foot, gill, mantle, palp, and siphon of Perkinsus-infected Manila clams. Furthermore, MCL3 was detected in the foot, gill, mantle, gonad, digestive gland, gonad connective tissue, intestine, and stomach by immunohistochemical localization.

Citations

Jul 23, 2013·Journal of Invertebrate Pathology·Philippe SoudantAswani Volety
Sep 10, 2013·Biochemistry. Biokhimii︠a︡·O V ChernikovP A Lukyanov
Feb 7, 2016·Fish & Shellfish Immunology·Mausumi Adhya, Biswajit Singha
Sep 29, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mausumi AdhyaMoonjae Cho
Feb 3, 2009·Developmental and Comparative Immunology·Huan ZhangLimei Qiu
Oct 23, 2009·Carbohydrate Research·Mausumi AdhyaBishnu P Chatterjee
Jul 27, 2015·Journal of Invertebrate Pathology·Tal Ben-HorinDavid Bushek
Feb 24, 2015·Fish & Shellfish Immunology·Marco Gerdol, Paola Venier
Mar 8, 2018·International Journal of Molecular Sciences·Weilin WangLinsheng Song

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