Characterized non-transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses

PloS One
Virginia MedinaJorge A Zavala

Abstract

The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro β-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs´ NTM microbiota may impact on nutrit...Continue Reading

References

Jan 1, 1987·Applied and Environmental Microbiology·G Kouker, K E Jaeger
Jun 1, 1984·Japanese Journal of Medical Science & Biology·Y KosakoE Yoshizaki
Jan 11, 2000·AJR. American Journal of Roentgenology·P D Bergey, L Axel
Jul 15, 2003·Journal of Chemical Ecology·Giorla Carla PiubelliFernando Mesquita Lara
Dec 4, 2003·Annual Review of Entomology·R J Dillon, V M Dillon
Oct 13, 2006·PLoS Biology·Takahiro HosokawaTakema Fukatsu
Jun 15, 2007·Proceedings. Biological Sciences·Takahiro HosokawaTakema Fukatsu
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jorge A ZavalaMay R Berenbaum
Oct 2, 2009·Environmental Entomology·Simone S PradoRodrigo P P Almeida
Jun 4, 2010·PloS One·Jonathan G Lundgren, R Michael Lehman
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yoshitomo KikuchiTakema Fukatsu
Jun 12, 2012·BMC Microbiology·Tiago D ZucchiFernando L Cônsoli
Sep 18, 2012·Molecular Ecology·D R ColmanC D Takacs-Vesbach
May 23, 2013·FEMS Microbiology Reviews·Philipp Engel, Nancy A Moran
Jun 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Chia-Ching ChuManfredo J Seufferheld
Sep 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Seung Ho ChungGary W Felton
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jan 5, 2014·Molecular Ecology·Parris T HumphreyNoah K Whiteman
Oct 24, 2014·Annual Review of Entomology·Angela E Douglas
Nov 12, 2014·Journal of Experimental Botany·Akiko SugioJean-Christophe Simon
Jul 15, 2015·Nature Communications·Javier A Ceja-NavarroEoin L Brodie
May 4, 2016·Nature Reviews. Microbiology·Waldan K Kwong, Nancy A Moran
Aug 5, 2017·Nature·Kevin R FosterSeth Rakoff-Nahoum

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Citations

Apr 11, 2020·Microbiology Resource Announcements·Desiree J MeyersWilliam B Whitman
Jan 13, 2021·Pest Management Science·Vanesa Gisela JacobiJorge Alberto Zavala

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Datasets Mentioned

BETA
ATCC
11775

Methods Mentioned

BETA
PCR
electrophoresis
dissection
ARISA

Software Mentioned

MALDI BiotyperTM
BioEdit Sequence Alignment Editor
GraphPad
MEGA
FlexControl
Muscle
BLAST
ARISA
Prism

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