Characterizing Antimicrobial Resistant Escherichia coli and Associated Risk Factors in a Cross-Sectional Study of Pig Farms in Great Britain.

Frontiers in Microbiology
Manal AbuOunM F Anjum

Abstract

Combatting antimicrobial resistant (AMR) using a One-Health approach is essential as various bacteria, including Escherichia coli, a common bacteria, are becoming increasingly resistant and livestock may be a reservoir. The AMR gene content of 492 E. coli, isolated from 56 pig farms across Great Britain in 2014-2015, and purified on antibiotic selective and non-selective plates, was determined using whole genome sequencing (WGS). The E. coli were phylogenetically diverse harboring a variety of AMR profiles with widespread resistance to "old" antibiotics; isolates harbored up to seven plasmid Inc-types. None showed concurrent resistance to third-generation cephalosporins, fluoroquinolones and clinically relevant aminoglycosides, although ∼3% harbored AMR genes to both the former two. Transferable resistance to carbapenem and colistin were absent, and six of 117 E. coli STs belonged to major types associated with human disease. Prevalence of genotypically MDR E. coli, gathered from non-selective media was 46.9% and that of extended-spectrum-beta-lactamase E. coli was low (∼4% from non-selective). Approximately 72.6% of E. coli from ciprofloxacin plates and only 8.5% from the other plates harbored fluoroquinolone resistance due to...Continue Reading

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Citations

Aug 12, 2020·The Journal of Antimicrobial Chemotherapy·Valeria BortolaiaFrank M Aarestrup
Mar 3, 2021·The ISME Journal·William MatlockUNKNOWN REHAB consortium

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Datasets Mentioned

BETA
MG1655
U00096.2
PRJEB26317

Software Mentioned

RaxML
APHA
NG
EvolView
Abricate
SeqFinder
R
AWERB
APHA SeqFinder
SPAdes

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