Characterizing breast cancer xenograft epidermal growth factor receptor expression by using near-infrared optical imaging.

Acta Radiologica
Kezheng WangXiaoyuan Chen

Abstract

Epidermal growth factor receptor (EGFR) overexpression is associated with several key features of cancer development and growth. Therefore, EGFR is a very promising biological target for tumor diagnosis and anticancer therapy. Characterization of EGFR expression is important for clinicians to select patients for EGFR-targeted therapy and evaluate therapeutic effects. To investigate whether near-infrared (NIR) fluorescent dye Cy5.5-labeled anti-EGFR monoclonal antibody Erbitux can characterize EGFR expression level in MDA-MB-231 and MCF-7 breast cancer xenografts using an in vivo NIR imaging method. A fluorochrome probe was designed by coupling Cy5.5 to Erbitux through acidylation, and the fluorescence property of the Erbitux-Cy5.5 conjugate was characterized by fluorospectroscopy. Flow cytometry and laser confocal microscopy were used to test the EGFR specificity of the antibody probe in vitro. Erbitux-Cy5.5 was also injected intravenously into immune-deficient mice bearing MDA-MB-231 or MCF-7 tumors. Whole-body and region-of-interest fluorescence images were acquired and analyzed. The EGFR expression was also analyzed and confirmed by immunohistochemical assay. The maximum excitation/emission wavelength for the Erbitux-Cy5.5 p...Continue Reading

Citations

Mar 9, 2017·The Journal of Pharmacy and Pharmacology·Sally SafwatNahed D Mortada
Dec 3, 2016·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Kimberley S SamkoeBrian W Pogue
Sep 21, 2010·Molecular Oncology·Jason R McCarthyRalph Weissleder
Nov 29, 2011·International Journal of Cancer. Journal International Du Cancer·Rana FilfilAnne E G Lenferink
Aug 19, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Baozhong ShenWu Lina
Jun 20, 2012·Journal of Biophotonics·Susan M Daly, Martin J Leahy
May 5, 2016·Oncotarget·Cristina MartelliLuisa Ottobrini
Jan 20, 2011·Contrast Media & Molecular Imaging·Yang LiuHong Zhang
Jul 19, 2016·International Journal of Cancer. Journal International Du Cancer·Mara SaccomanoJoanna Napp
Sep 1, 2017·Journal of the American Oil Chemists' Society·Ahmed Abu-Fayyad, Sami Nazzal
Dec 25, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Alaadin Y AlayoubiSami Nazzal
Jan 20, 2021·ACS Biomaterials Science & Engineering·Johana UribeSusan Daniel

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