Characterizing cellular mechanical phenotypes with mechano-node-pore sensing

Microsystems & Nanoengineering
Junghyun KimLydia L Sohn

Abstract

The mechanical properties of cells change with their differentiation, chronological age, and malignant progression. Consequently, these properties may be useful label-free biomarkers of various functional or clinically relevant cell states. Here, we demonstrate mechano-node-pore sensing (mechano-NPS), a multi-parametric single-cell-analysis method that utilizes a four-terminal measurement of the current across a microfluidic channel to quantify simultaneously cell diameter, resistance to compressive deformation, transverse deformation under constant strain, and recovery time after deformation. We define a new parameter, the whole-cell deformability index (wCDI), which provides a quantitative mechanical metric of the resistance to compressive deformation that can be used to discriminate among different cell types. The wCDI and the transverse deformation under constant strain show malignant MCF-7 and A549 cell lines are mechanically distinct from non-malignant, MCF-10A and BEAS-2B cell lines, and distinguishes between cells treated or untreated with cytoskeleton-perturbing small molecules. We categorize cell recovery time, ΔTr, as instantaneous (ΔTr ~ 0 ms), transient (ΔTr ≤ 40ms), or prolonged (ΔTr > 40ms), and show that the com...Continue Reading

References

Mar 23, 1987·FEBS Letters·M CouéE D Korn
Nov 1, 1973·Journal of the National Cancer Institute·D J GiardW P Parks
Dec 1, 1981·The American Journal of Medicine·D L DexterP Calabresi
May 27, 1999·Journal of Biochemical and Biophysical Methods·O ThoumineJ J Meister
Dec 28, 1999·Journal of Biomechanics·R M Hochmuth
May 12, 2000·American Journal of Respiratory and Critical Care Medicine·L A KirschenbaumE C Rackow
Jun 15, 2000·Nature Cell Biology·W M MortonP J McLaughlin
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Omar A Saleh, Lydia L Sohn
Mar 1, 2003·Biophysical Journal·Jordi AlcarazDaniel Navajas
Oct 29, 2004·The American Journal of Pathology·Alvin Y LiuLawrence D True
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Stephane Brunet, Bernard Maro
Jun 30, 2006·Journal of Mammary Gland Biology and Neoplasia·Thorarinn GudjonssonMina J Bissell
Jan 22, 2008·Experimental Gerontology·Catharina Bertram, Ralf Hass
Apr 2, 2008·IUBMB Life·Eddy Pasquier, Maria Kavallaris
Jun 28, 2008·Lab on a Chip·Michael J RosenbluthDaniel A Fletcher
Jul 29, 2008·Biochemical and Biophysical Research Communications·Q S LiC T Lim
Jan 21, 2009·Cancer Metastasis Reviews·Sanjay Kumar, Valerie M Weaver
Sep 11, 2009·Current Opinion in Cell Biology·Akiko Mammoto, Donald E Ingber
Nov 10, 2009·Ageing Research Reviews·Maria N Starodubtseva
Dec 30, 2009·Electrophoresis·Jinwen ZhouNicolas Hans Voelcker
Feb 12, 2010·Nature Reviews. Cancer·Maria Kavallaris
Apr 27, 2011·Journal of Cellular Physiology·Marta De DonatoCristiano Ferlini
Jun 28, 2011·Nature Reviews. Cancer·Denis WirtzPeter C Searson
Oct 4, 2011·The Journal of Clinical Investigation·Kornelia Polyak
May 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Daniel R GossettDino Di Carlo
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Rafael D González-CruzEric M Darling
Oct 3, 2012·Journal of the Mechanical Behavior of Biomedical Materials·William RonanJ Patrick McGarry
Jan 10, 2013·British Journal of Cancer·R FisherC Swanton
Jan 19, 2013·Journal of Visualized Experiments : JoVE·Mark A LabargeMartha R Stampfer
Feb 7, 2013·Lab on a Chip·Karthik R BalakrishnanLydia L Sohn
Apr 13, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Zhuolong ZhouAnxun Wang
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sangwon ByunScott R Manalis
Jul 26, 2013·Lab on a Chip·Jaideep S DudaniDino Di Carlo

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Citations

Dec 21, 2018·Lab on a Chip·Antoine Leblanc-HotteYves-Alain Peter
Jun 23, 2020·Biomicrofluidics·Molly Kozminsky, Lydia L Sohn
Apr 25, 2018·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Thomas R CareyLydia L Sohn
Jul 20, 2019·Oxidative Medicine and Cellular Longevity·Sashko G SpassovStefan Schumann
Oct 22, 2020·Journal of Nanobiotechnology·Ines LüchtefeldMassimo Vassalli
Apr 10, 2021·The Journal of Chemical Physics·Wilfred Shelby Russell, Zuzanna Siwy
Jul 23, 2021·Progress in Biophysics and Molecular Biology·Rekha GovindanSabyasachi Senapati
Nov 9, 2018·Analytical Chemistry·Gongchen Sun, Hang Lu

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Methods Mentioned

BETA
biopsy
AFM
Fluorescence
confocal microscopy
FACS

Software Mentioned

NPS

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