Characterizing fibroblast migration on discrete collagen threads for applications in tissue regeneration

Journal of Biomedical Materials Research. Part a
Kevin G CornwellGeorge D Pins

Abstract

Collagen threads with mechanical properties and fibrillar substructure similar to native tissue have been synthesized for the repair of injured tendon and ligament. While these scaffolding materials have demonstrated the potential for inducing tissue regeneration, one limitation has been an insufficient rate of tissue ingrowth for complete regeneration. We hypothesize that the structural hierarchy and biochemical cues on the surfaces of these threads will enhance the rate of cell migration and ultimately the rate of new tissue ingrowth. We developed an in vitro assay to measure the effects of various collagen sources and crosslinking on the rate of fibroblast migration on the surfaces of collagen threads. Threads were suspended from elevated platforms and seeded with fibroblast-populated collagen lattices. Cell migration rates ranging from 0.75 to 1.25 mm/day were measured as the fibroblasts left the lattices and migrated onto various thread types. Threads self-assembled from type I collagen were found to have migration rates similar to native tendon threads while crosslinking by severe dehydration decreased the rate. This novel in vitro model system allows examination of cell migration from a wound margin onto biomaterials to ...Continue Reading

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Citations

Jul 14, 2005·Clinical Obstetrics and Gynecology·Jeffrey L Cornella
Jan 22, 2009·Journal of Biomaterials Science. Polymer Edition·D I ZeugolisG Attenburrow
Aug 27, 2014·Stem Cell Research & Therapy·Sunny Akogwu AbbahDimitrios I Zeugolis
May 1, 2012·Cirugía española·Manuel López CanoMaría Antonia Arbós Vía
Sep 26, 2009·Clinics in Podiatric Medicine and Surgery·Kevin G CornwellKenneth S James
Jul 30, 2015·Acta Biomaterialia·Jonathan M GrasmanGeorge D Pins
Sep 11, 2012·Journal of Biomedical Materials Research. Part a·Jacques P GuyetteGlenn R Gaudette
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Sep 20, 2006·Biomaterials·Derek M DoroskiJohnna S Temenoff
May 9, 2015·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Mohammad SanamiDimitrios I Zeugolis
Oct 27, 2015·Biotechnology and Bioengineering·Dilip ThomasDimitrios I Zeugolis
Dec 17, 2015·Advanced Healthcare Materials·Sunny A AbbahDimitrios I Zeugolis
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Sep 27, 2006·Journal of Biomedical Materials Research. Part a·Kevin G CornwellGeorge D Pins
Oct 3, 2006·Journal of Biomedical Materials Research. Part a·Katie A BushGeorge D Pins
Oct 25, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·D I ZeugolisG Attenburrow
May 10, 2008·Journal of Biomedical Materials Research. Part a·Dimitrios I ZeugolisGeoffrey Attenburrow
Apr 10, 2013·Journal of Biomaterials Science. Polymer Edition·Tomoko HashimotoYasushi Tamada

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