Characterizing gibberellin flow in planta using photocaged gibberellins

Chemical Science
Shira WexlerRoy Weinstain

Abstract

Gibberellins (GAs) are ubiquitous plant hormones that coordinate central developmental and adaptive growth processes in plants. Accurate movement of GAs throughout the plant from their sources to their destination sites is emerging to be a highly regulated and directed process. We report on the development of novel photocaged gibberellins that, in combination with a genetically encoded GA-response marker, provide a unique platform to study GA movement at high-resolution, in real time and in living, intact plants. By applying this platform to the Arabidopsis thaliana endogenous bioactive gibberellin GA4, we measure kinetic parameters of its flow, such as decay length and velocity, in vivo.

References

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Citations

Nov 24, 2019·Proceedings of the National Academy of Sciences of the United States of America·Juan-José RipollAdrienne H K Roeder
Jul 12, 2020·Plant & Cell Physiology·Peter Hedden
Jul 27, 2021·Chemistry : a European Journal·C-C George YehSam P de Visser
Oct 31, 2020·Chemical Reviews·Roy WeinstainPetr Klán
Oct 7, 2021·Journal of Agricultural and Food Chemistry·Noemi HemelíkováVáclav Mik

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Methods Mentioned

BETA
transgenic
two-hybrid
Fluorescence

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