Characterizing non-photochemical quenching in leaves through fluorescence lifetime snapshots

Photosynthesis Research
Emily J Sylak-GlassmanGraham R Fleming

Abstract

We describe a technique to measure the fluorescence decay profiles of intact leaves during adaptation to high light and subsequent relaxation to dark conditions. We show how to ensure that photosystem II reaction centers are closed and compare data for wild type Arabidopsis thaliana with conventional pulse-amplitude modulated (PAM) fluorescence measurements. Unlike PAM measurements, the lifetime measurements are not sensitive to photobleaching or chloroplast shielding, and the form of the fluorescence decay provides additional information to test quantitative models of excitation dynamics in intact leaves.

References

May 30, 1973·Biochimica Et Biophysica Acta·A Y Borisov, M D Il'ina
Jul 6, 2002·Science·Carsten KülheimStefan Jansson
May 1, 2008·Annual Review of Plant Biology·Neil R Baker
Mar 3, 2010·Annual Review of Plant Biology·Xin-Guang ZhuDonald R Ort
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kapil AmarnathGraham R Fleming
Nov 26, 2014·Proceedings of the National Academy of Sciences of the United States of America·Emily J Sylak-GlassmanGraham R Fleming

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Citations

Jan 21, 2016·Proceedings of the National Academy of Sciences of the United States of America·Kapil AmarnathGraham R Fleming
Oct 24, 2019·Annals of Botany·Alexandrina StirbetGovindjee Govindjee
Sep 22, 2018·Proceedings of the National Academy of Sciences of the United States of America·Doran I G BennettKapil Amarnath
Nov 28, 2018·Photosynthesis Research·Volha U ChukhutsinaRoberta Croce
Apr 4, 2018·Nature Plants·Shazia FarooqHerbert van Amerongen
Nov 18, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Herbert van Amerongen, Jevgenij Chmeliov
Jun 19, 2021·Annual Review of Plant Biology·Roberto Bassi, Luca Dall'Osto
Oct 31, 2017·The Journal of Physical Chemistry Letters·Soomin ParkGraham R Fleming

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