Characterizing recombinant proteins using HPLC gel filtration and mass spectrometry

Current Protocols in Protein Science
G E BeggD W Speicher

Abstract

Recombinant proteins are subject to many forms of heterogeneity, including aggregation, proteolytic degradation, chemical modification, mutation, and incorrect translation. This unit describes methods for the detection and identification of these problems using analytical HPLC gel filtration and MALDI-MS. Preliminary characterization of recombinants is necessary before the structure or function of the protein can be investigated.

Citations

May 9, 2014·PloS One·Małgorzata KozłowskaAndrzej Ożyhar
Oct 13, 2010·The Journal of Cell Biology·Damien GarbettAnthony Bretscher

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