Characterizing the Circulating Cell Populations in Traumatic Heterotopic Ossification

The American Journal of Pathology
Shawn J LoderBenjamin Levi

Abstract

Heterotopic ossification (HO) occurs secondary to trauma, causing pain and functional limitations. Identification of the cells that contribute to HO is critical to the development of therapies. Given that innate immune cells and mesenchymal stem cells are known contributors to HO, we sought to define the contribution of these populations to HO and to identify what, if any, contribution circulating populations have to HO. A shared circulation was obtained using a parabiosis model, established between an enhanced green fluorescent protein-positive/luciferase+ donor and a same-strain nonreporter recipient mouse. The nonreporter mouse received Achilles tendon transection and dorsal burn injury to induce HO formation. Bioluminescence imaging and immunostaining were performed to define the circulatory contribution of immune and mesenchymal cell populations. Histologic analysis showed circulating cells present throughout each stage of the developing HO anlagen. Circulating cells were present at the injury site during the inflammatory phase and proliferative period, with diminished contribution in mature HO. Immunostaining demonstrated that most early circulatory cells were from the innate immune system; only a small population of mese...Continue Reading

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Citations

Mar 4, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shanrun LiuThomas M Ryan
Jan 9, 2020·Clinical Orthopaedics and Related Research·S Peter MagnussonPernilla Eliasson
Sep 10, 2020·Nature Reviews. Molecular Cell Biology·Ankit SalhotraMichael T Longaker
Jun 3, 2020·JCI Insight·Ginny Ching-Yun HsuAaron W James
Feb 20, 2021·Stem Cell Reports·Chase A PaganiBenjamin Levi
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Dorothée GirardSébastien Banzet
Jul 1, 2021·Journal of Cellular and Molecular Medicine·Bing TuCunyi Fan

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