Characterizing the flagellar filament and the role of motility in bacterial prey-penetration by Bdellovibrio bacteriovorus

Molecular Microbiology
Carey LambertR Elizabeth Sockett

Abstract

The predatory bacterium Bdellovibrio bacteriovorus swims rapidly by rotation of a single, polar flagellum comprised of a helical filament of flagellin monomers, contained within a membrane sheath and powered by a basal motor complex. Bdellovibrio collides with, enters and replicates within bacterial prey, a process previously suggested to firstly require flagellar motility and then flagellar shedding upon prey entry. Here we show that flagella are not always shed upon prey entry and we study the six fliC flagellin genes of B. bacteriovorus, finding them all conserved and expressed in genome strain HD100 and the widely studied lab strain 109J. Individual inactivation of five of the fliC genes gave mutant Bdellovibrio that still made flagella, and which were motile and predatory. Inactivation of the sixth fliC gene abolished normal flagellar synthesis and motility, but a disordered flagellar sheath was still seen. We find that this non-motile mutant was still able to predate when directly applied to lawns of YFP-labelled prey bacteria, showing that flagellar motility is not essential for prey entry but important for efficient encounters with prey in liquid environments.

References

May 1, 1990·Journal of Bacteriology·John J TudorI A Acrich
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D N Arnosti, M J Chamberlin
Dec 1, 1986·Molecular & General Genetics : MGG·M RogersD Sherratt
Sep 1, 1985·Journal of Bacteriology·L S Thomashow, S C Rittenberg
Sep 1, 1985·Journal of Bacteriology·L S Thomashow, S C Rittenberg
Jan 1, 1972·Journal of Bacteriology·S C Rittenberg
Nov 1, 1969·Journal of Bacteriology·R J Seidler, M P STARR
Jan 1, 1969·Current Topics in Microbiology and Immunology·M Shilo
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Aug 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·L L McCarter

Citations

Dec 22, 2009·Current Microbiology·Carey LambertR Elizabeth Sockett
Nov 30, 2012·The ISME Journal·Zohar PasternakEdouard Jurkevitch
May 8, 2008·Nature Reviews. Microbiology·Ken F Jarrell, Mark J McBride
Jul 16, 2008·Applied and Environmental Microbiology·John J TudorAdam Zwolak
Jun 28, 2011·Applied and Environmental Microbiology·Robert J AtterburyR Elizabeth Sockett
Jun 15, 2007·Applied and Environmental Microbiology·Susan R Steyert, Silvia A Pineiro
Feb 8, 2011·Applied and Environmental Microbiology·Kaoru UchidaShin-Ichi Aizawa
Apr 26, 2011·Journal of Bacteriology·Carey LambertR Elizabeth Sockett
Dec 22, 2009·Journal of Bacteriology·Andrew K FentonR Elizabeth Sockett
Mar 29, 2011·Journal of Bacteriology·Alexandra Faulds-PainPhillip D Aldridge
Feb 1, 2011·Journal of Bacteriology·Nicole RoschanskiEckhard Strauch
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Kevin D Young
Jul 7, 2009·Annual Review of Microbiology·R Elizabeth Sockett
Dec 15, 2010·BMC Research Notes·Jonathan Ferooz, Jean-Jacques Letesson
Jan 12, 2010·PloS One·Carey LambertR Elizabeth Sockett
Jul 31, 2007·Future Microbiology·Eckhard StrauchMichael Linscheid
Apr 10, 2007·Journal of Bacteriology·Katy J EvansR Elizabeth Sockett
Jul 3, 2007·Journal of Bacteriology·Cecilia M ArraianoJörg Vogel
Feb 13, 2016·Proceedings. Biological Sciences·Felix J H HolDaniel A Koster
Oct 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Or RotemEdouard Jurkevitch
Feb 19, 2009·Research in Microbiology·Adrian A Medina, D E Kadouri
Nov 7, 2015·Seminars in Cell & Developmental Biology·Bonnie ChabanMorgan Beeby
Oct 24, 2006·Current Opinion in Microbiology·Carey LambertR Elizabeth Sockett
May 14, 2011·Molecular Microbiology·Gladys AlexandreTarek Msadek
Mar 29, 2014·Environmental Microbiology·Mohammed DwidarRobert J Mitchell
Jun 13, 2009·FEMS Microbiology Reviews·James E Berleman, John R Kirby
Oct 22, 2008·Advances in Microbial Physiology·Carey LambertLiz Sockett
Dec 5, 2016·FEMS Microbiology Ecology·Sally OttoLukas Y Wick
Aug 16, 2017·Physical Biology·Hossein JashnsazSteve Pressé
Sep 2, 2015·Journal of Bacteriology·Or RotemEdouard Jurkevitch
May 12, 2019·Applied and Environmental Microbiology·Łukasz MakowskiJolanta Zakrzewska-Czerwińska
Sep 10, 2019·Environmental Microbiology·Hansol ImRobert J Mitchell
Dec 12, 2012·Environmental Microbiology·Virginia MartínezMaría Auxiliadora Prieto
Jul 25, 2019·Biomolecules·Shuichi Nakamura, Tohru Minamino
May 29, 2019·Environmental Microbiology·Rajesh SathyamoorthyEdouard Jurkevitch
Mar 10, 2020·FEMS Microbiology Reviews·Morgan BeebyDavid R Mitchell
Sep 5, 2020·The ISME Journal·Rajesh SathyamoorthyEdouard Jurkevitch
Apr 27, 2017·FEMS Microbiology Letters·Julia JohnkeAntonis Chatzinotas
Dec 1, 2020·Molecular Microbiology·Bailey Milne-DaviesAndreas Diepold
Sep 23, 2019·Biochemical and Biophysical Research Communications·So Yeon ChoSung-Il Yoon
Apr 16, 2021·World Journal of Microbiology & Biotechnology·Monique WasoWesaal Khan
May 4, 2021·Critical Reviews in Microbiology·Francis M CavalloJan Maarten van Dijl

Datasets Mentioned

BETA
AJ851165

Methods Mentioned

BETA
electron microscopy
PCR
reverse transcription PCR

Related Concepts

Bdellovibrio
Cell Motility
Flagella
Flagellin
Genes, Bacterial
Genome, Bacterial

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