PMID: 12770226May 29, 2003Paper

Characterizing the Hez-PBAN gene products in neuronal clusters with immunocytochemistry and MALDI MS

Journal of Insect Physiology
P W.K. MaW L Roelofs

Abstract

Methods to characterize pheromone biosynthesis activating neuropeptide (PBAN) and other PBAN gene encoded neuropeptides (PGN) from individual subesophageal ganglion neuronal clusters of the corn earworm moth, Helicoverpa zea, were developed. Individual antisera against the N-terminal sequence to PBAN and each of the three PGNs from the Hez-PBAN prohormone were developed, and their specificity determined. In all cases, each antiserum stains the same three groups of subesophageal ganglion ventral midline neurons-the mandibular, maxillary and labial neurons-in both adult females and males. These results were confirmed using matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) of individual subesophageal ganglion neuronal clusters. Using mass spectrometry, the amidated PGN-24 was not detected but an N-terminally extended form is observed that is two amino acids longer. Other peptides resulting from the processing of the Hez-PBAN prohormone were detected. Using both the specific antisera and the cellular profiling abilities of MALDI MS, the roles of individual members of the Hez-PBAN prohormone derived peptides can now be explored.

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Citations

Mar 31, 2000·Trends in Biotechnology·L LiJ V Sweedler
Aug 19, 2007·Nature Protocols·Stanislav S Rubakhin, Jonathan V Sweedler
Apr 11, 2014·PloS One·Man-Yeon ChoiReinhard Predel
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoonseong ParkMichael E Adams
May 23, 2008·General and Comparative Endocrinology·Ada Rafaeli
Jun 7, 2005·Mass Spectrometry Reviews·Amanda B HummonJonathan V Sweedler
Oct 18, 2011·Expert Review of Proteomics·Kristin J BoggioJeffrey N Agar
Oct 30, 2012·Journal of Insect Physiology·Guy BlochAda Rafaeli
Apr 29, 2010·Chemical Reviews·Kamila Chughtai, Ron M A Heeren
Aug 19, 2008·Analytical Chemistry·Stanislav S Rubakhin, Jonathan V Sweedler
Sep 26, 2008·Journal of Proteome Research·Adriana BoraJonathan V Sweedler

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