Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility

PloS One
L L SchumacherC K Jones

Abstract

New regulatory and consumer demands highlight the importance of animal feed as a part of our national food safety system. Porcine epidemic diarrhea virus (PEDV) is the first viral pathogen confirmed to be widely transmissible in animal food. Because the potential for viral contamination in animal food is not well characterized, the objectives of this study were to 1) observe the magnitude of virus contamination in an animal food manufacturing facility, and 2) investigate a proposed method, feed sequencing, to decrease virus decontamination on animal food-contact surfaces. A U.S. virulent PEDV isolate was used to inoculate 50 kg swine feed, which was mixed, conveyed, and discharged into bags using pilot-scale feed manufacturing equipment. Surfaces were swabbed and analyzed for the presence of PEDV RNA by quantitative real-time polymerase chain reaction (qPCR). Environmental swabs indicated complete contamination of animal food-contact surfaces (0/40 vs. 48/48, positive baseline samples/total baseline samples, positive subsequent samples/total subsequent samples, respectively; P < 0.05) and near complete contamination of non-animal food-contact surfaces (0/24 vs. 16/18, positive baseline samples/total baseline samples, positive s...Continue Reading

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Citations

Sep 8, 2020·Animal Health Research Reviews·Savannah C StewartCassandra K Jones
Jul 27, 2020·Translational Animal Science·Marut SaensukjaroenphonCassandra K Jones
Dec 20, 2019·Veterinary Medicine and Science·Cassandra K JonesChad B Paulk
May 3, 2020·Transboundary and Emerging Diseases·Scott A DeePaul Sundberg
Jan 15, 2020·Journal of Veterinary Research·Marta Antas, Grzegorz Woźniakowski
Jun 26, 2021·Transboundary and Emerging Diseases·Gerald C ShursonJennifer L G van de Ligt
Sep 24, 2021·Transboundary and Emerging Diseases·Jordan T GebhardtJason C Woodworth

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Methods Mentioned

BETA
PCR

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