Charcot-Leyden crystal formation is closely associated with eosinophil extracellular trap cell death.

Blood
Shigeharu UekiP F Weller

Abstract

Protein crystallization in human tissue rarely occurs. Charcot-Leyden crystals (CLCs) were described in various eosinophilic diseases >150 years ago, but our understanding of CLC formation still remains limited. In this study, we demonstrate that CLCs observed in varied inflamed human tissues are closely associated with eosinophil cell-free granules and nuclear envelope/plasma membrane disintegration with release of filamentous chromatin (extracellular traps), typical morphologies of a regulated pathway of extracellular trap cell death (ETosis). During the process of eosinophil ETosis, eccentrically localized cytoplasmic and perinuclear CLC protein (galectin-10) is homogeneously redistributed in the cytoplasm. Rapid (1-2 minutes) formation of intracytoplasmic CLCs was observed using time-lapse imaging. Plasma membrane rupture enabled the release of both intracellularly formed CLCs and soluble galectin-10 that further contributed to formation of CLCs extracellularly, in parallel with the expulsion of free intact granules and extracellular traps. CLC formation and galectin-10 release were dependent on nicotinamide adenine dinucleotide phosphate oxidase activation. To our knowledge, this is the first demonstration of natural forma...Continue Reading

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Citations

Dec 19, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juan Francisco Rodríguez-AlcázarBernardo S Franklin
Nov 18, 2018·Blood·Amy D Klion
Oct 31, 2019·Traffic·Celestin N MudogoChristian Betzel
Oct 1, 2019·Current Opinion in Allergy and Clinical Immunology·Gwanghui Ryu, Dae Woo Kim
Jan 25, 2020·Annual Review of Pathology·Amy D KlionBruce S Bochner
Apr 10, 2020·Journal of Leukocyte Biology·Peter F WellerRossana C N Melo
May 22, 2020·Oral Diseases·Yoshinobu KawamuraShigeharu Ueki
Dec 12, 2018·Frontiers in Immunology·Manali MukherjeeShigeharu Ueki
Aug 14, 2020·Asia Pacific Allergy·Masahide TakedaMakoto Hirokawa
Jun 17, 2019·Current Allergy and Asthma Reports·Shigeharu UekiPeter F Weller
Aug 2, 2019·World Journal of Gastroenterology : WJG·Alexandre Loktionov
May 28, 2019·Science·Judith E Allen, Tara E Sutherland
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Aug 14, 2021·Asia Pacific Allergy·Konomi KobayashiUNKNOWN iPOT-5 Investigators
Apr 13, 2021·American Journal of Respiratory and Critical Care Medicine·Kota IkariShigeharu Ueki

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