Charge-based engineering of hydrophobin HFBI: effect on interfacial assembly and interactions

Biomacromolecules
Michael LienemannMarkus Linder

Abstract

Hydrophobins are extracellular proteins produced by filamentous fungi. They show a variety of functions at interfaces that help fungi to adapt to their environment by, for example, adhesion, formation of coatings, and lowering the surface tension of water. Hydrophobins fold into a globular structure and have a distinct hydrophobic patch on their surface that makes these proteins amphiphilic. Their amphiphilicity implies interfacial assembly, but observations indicate that intermolecular interactions also contribute to their functional properties. Here, we used the class II hydrophobin HFBI from Trichoderma reesei as a model to understand the structural basis for the function of hydrophobins. Four different variants were made in which charged residues were mutated. The residues were chosen to probe the role of different regions of the hydrophilic part of the proteins. Effects of the mutations were studied by analyzing the formation and structure of self-assembled layers, multimerization in solution, surface adhesion, binding of secondary layers of proteins on hydrophobins, and the viscoelastic behavior of the air-water interface during formation of protein films; the comparison showed clear differences between variants only in t...Continue Reading

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Citations

Apr 5, 2016·The Journal of Physical Chemistry. B·Ryota Yamasaki, Tetsuya Haruyama
Jun 1, 2016·Trends in Biochemical Sciences·Marieke SchorNicola R Stanley-Wall
Oct 1, 2016·The Journal of Physical Chemistry Letters·Konrad MeisterHuib J Bakker
Oct 16, 2016·Advanced Materials·Hendrik HählJean-Baptiste Fleury
Nov 11, 2016·PLoS Computational Biology·Laura Riccardi, Paolo Mereghetti
Mar 28, 2017·The Journal of Physical Chemistry Letters·Konrad MeisterHuib J Bakker
Oct 28, 2017·Applied Microbiology and Biotechnology·Xiangxiang WangMingqiang Qiao
Apr 18, 2017·Critical Reviews in Food Science and Nutrition·Christina Linke, Stephan Drusch
Jan 27, 2019·Journal of Biological Engineering·Bryan W Berger, Nathanael D Sallada
Jan 18, 2021·International Journal of Biological Macromolecules·Xiangxiang WangMingqiang Qiao
May 3, 2021·The Journal of Biological Chemistry·Arja PaananenAndreas Ebner
Oct 19, 2017·ACS Omega·Alessandra GriffoPäivi Laaksonen
Dec 27, 2017·Biochemistry·Nathanael D SalladaBryan W Berger
Jul 4, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Hendrik HählKarin Jacobs
Jun 12, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hendrik HählJean-Baptiste Fleury

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