Charge-Based Separation of Micro- and Nanoparticles

Micromachines
Bao D HoJonas O Tegenfeldt

Abstract

Deterministic Lateral Displacement (DLD) is a label-free particle sorting method that separates by size continuously and with high resolution. By combining DLD with electric fields (eDLD), we show separation of a variety of nano and micro-sized particles primarily by their zeta potential. Zeta potential is an indicator of electrokinetic charge-the charge corresponding to the electric field at the shear plane-an important property of micro- and nanoparticles in colloidal or separation science. We also demonstrate proof of principle of separation of nanoscale liposomes of different lipid compositions, with strong relevance for biomedicine. We perform careful characterization of relevant experimental conditions necessary to obtain adequate sorting of different particle types. By choosing a combination of frequency and amplitude, sorting can be made sensitive to the particle subgroup of interest. The enhanced displacement effect due to electrokinetics is found to be significant at low frequency and for particles with high zeta potential. The effect appears to scale with the square of the voltage, suggesting that it is associated with either non-linear electrokinetics or dielectrophoresis (DEP). However, since we observe large chang...Continue Reading

References

Jan 15, 1994·Journal of Biotechnology·G H MarkxR Pethig
Jul 2, 1999·Biophysical Journal·H MorganN G Green
Sep 2, 1999·Journal of Colloid and Interface Science·A RamosA Castellanos
Nov 23, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·N G GreenA Castellanos
Nov 23, 2000·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·A GonzalezH Morgan
Sep 21, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·N G GreenA Castellanos
Sep 27, 2002·Biophysical Journal·Chia-Fu ChouRobert H Austin
Mar 5, 2004·Physical Review Letters·Martin Z Bazant, Todd M Squires
Mar 11, 2004·IEEE Engineering in Medicine and Biology Magazine : the Quarterly Magazine of the Engineering in Medicine & Biology Society·Kai F HoettgesMartin B McDonnell
Mar 17, 2004·Analytical Chemistry·Blanca H Lapizco-EncinasYolanda Fintschenko
May 15, 2004·Science·Lotien Richard HuangJames C Sturm
Jun 10, 2004·Electrophoresis·Blanca H Lapizco-EncinasYolanda Fintschenko
Mar 31, 2005·Journal of Colloid and Interface Science·Irina Ermolina, Hywel Morgan
Jul 27, 2005·Journal of Structural Biology·I F Sbalzarini, P Koumoutsakos
Sep 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·John A DavisRobert H Austin
May 10, 2007·Analytical Chemistry·Michele D Pysher, Mark A Hayes
Apr 29, 1966·Science·H A Pohl, I Hawk
Sep 28, 2007·Biomedical Microdevices·Yuejun KangJosiane E Eid
Feb 19, 2008·Analytical Chemistry·Kai F HoettgesMichael P Hughes
Feb 20, 1995·Biotechnology and Bioengineering·G H Markx, R Pethig
Aug 26, 2009·Lab on a Chip·Jason P BeechJonas O Tegenfeldt
Sep 29, 2009·Journal of Colloid and Interface Science·Junjie Zhu, Xiangchun Xuan
Oct 9, 2009·Analytical Chemistry·James V GreenShashi K Murthy
Jan 1, 2009·Biomicrofluidics·Christopher ChurchXiangchun Xuan
Feb 19, 2011·Lab on a Chip·Stefan H HolmJonas O Tegenfeldt
Feb 9, 2012·Lab on a Chip·William A BraffCullen R Buie
Feb 14, 2012·Lab on a Chip·Jason P BeechJonas O Tegenfeldt
Nov 1, 2012·AIP Advances·Kevin LoutherbackJames C Sturm
Feb 26, 2013·Nanoscale Research Letters·Abolfazl AkbarzadehKazem Nejati-Koshki
Feb 27, 2013·Sensors and Actuators. B, Chemical·Fernanda Camacho-AlanisAlexandra Ros
Sep 6, 2013·ACS Nano·Bahige G AbdallahAlexandra Ros
Mar 1, 2014·Nature Protocols·Nezihi Murat KarabacakMehmet Toner
Sep 13, 2014·Lab on a Chip·J McGrathH Bridle
Sep 26, 2015·Scientific Reports·Zongbin LiuLidong Qin
Nov 18, 2015·Lab on a Chip·Kerwin Kwek ZemingChia-Hung Chen

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

ImageJ
eDLD

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