Charge compensated binding of divalent metals to bacterioferritin: H+ release associated with cobalt(II) and zinc(II) binding at dinuclear metal sites

FEBS Letters
Nick E Le BrunG R Moore

Abstract

Divalent metal ion binding to the bacterial iron-storage protein, bacterioferritin (BFR), which contains a dinuclear metal binding site within each of its 24 subunits, was investigated by potentiometric and spectrophotometric methods. Cobalt(II) and zinc(II) were found to bind at both high- and low-affinity sites. Cobalt(II) binding at the high-affinity site was observed at a level of two per subunit with the release of approximately 1.6 protons per metal ion, thus confirming the dinuclear metal centre as the high-affinity site. Zinc(II) binding at the dinuclear centre (high-affinity site) resulted in the release of approximately 2 protons per metal ion, but exhibited a binding stoichiometry which indicated that not all dinuclear centres were capable of binding two zinc(II) ions. Competition data showed that binding affinities for the dinuclear centre were in the order zinc(II) > cobalt(II), and also confirmed the unexpected stoichiometry of zinc(II) binding. This work emphasises the importance of charge neutrality at the dinuclear centre.

References

Dec 11, 1991·Biochimica Et Biophysica Acta·E R BaumingerA Treffry
Jan 31, 1989·Biochemical and Biophysical Research Communications·S C AndrewsP M Harrison
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G D WattG C Papaefthymiou
Jan 1, 1972·Methods in Enzymology·M Laskowski, W R finkenstadt
Jul 1, 1981·The Biochemical Journal·J YarivS Ofer
Jul 1, 1994·Nature Structural Biology·F FrolowJ Yariv
Dec 1, 1995·The Biochemical Journal·N E Le BrunA J Thomson

❮ Previous
Next ❯

Citations

Feb 9, 2005·Biopolymers·Jennifer R CalhounWilliam F DeGrado
Aug 11, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ornella MaglioAngela Lombardi
Mar 13, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Rafael Torres Martin de RosalesAngela Lombardi
Jun 29, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Kourosh Honarmand EbrahimiWilfred R Hagen
Dec 4, 2003·Journal of Molecular Biology·Jennifer R CalhounJeffery G Saven
Sep 11, 2002·Journal of Molecular Biology·Christopher M SummaWilliam F DeGrado
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Murielle Rabiller-Baudry, Bernard Chaufer
Apr 24, 2001·Protein Science : a Publication of the Protein Society·A PasternakW F Degrado
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·A LombardiW F DeGrado
Dec 16, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Helen Aitken-RogersNick E Le Brun
Nov 1, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Allison LewinNick E Le Brun
Apr 15, 2010·Biochimica Et Biophysica Acta·Nick E Le BrunGeoffrey R Moore

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.