Charge-directed targeting of antimicrobial protein-nanoparticle conjugates

Biotechnology and Bioengineering
Rohan Satishkumar, Alexey Vertegel

Abstract

Use of antimicrobial enzymes covalently attached to nanoparticles is of great interest as an antibiotic-free approach to treat microbial infections. Intrinsic properties of nanoparticles can also be used to add functionality to their conjugates with biomolecules. Here, we show in a model system that nanoparticle charge can be used to enhance delivery and increase bactericidal activity of an antimicrobial enzyme, lysozyme. Hen egg lysozyme was covalently attached to two types of polystyrene latex nanoparticles: positively charged, containing aliphatic amine surface groups, and negatively charged, containing sulfate and chloromethyl surface groups. In the case of bacterial lysis assay with a Gram-positive bacteria Micrococcus lysodeikticus, activity of lysozyme conjugated to positively charged nanoparticles was approximately twice as large as that of free lysozyme, while lysozyme conjugated to negatively charged nanoparticles showed little detectable activity. At the same time, when assayed using a low-molecular weight oligosaccharide substrate, lysozyme attached to both positively and negatively charged nanoparticles showed slightly lower activity than free enzyme. A possible explanation of these results is that lysozyme attache...Continue Reading

References

Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M R PincusH A Scheraga
Sep 1, 1991·The Journal of Infectious Diseases·J D CoonrodK Yoneda
Jun 1, 1967·Journal of Bacteriology·C Cutinelli, F Galdiero
Apr 22, 1969·Biochimica Et Biophysica Acta·R C Davies, A Neuberger
Aug 1, 1993·Applied Microbiology and Biotechnology·A KondoK Higashitani
Jul 1, 1996·Pharmaceutical Research·O B KinstlerM J Treuheit
Jul 20, 1999·Ocular Immunology and Inflammation·A J KlaegerR S Stephens
Oct 26, 2000·Journal of Colloid and Interface Science·C E GiacomelliW Norde
Nov 23, 2000·Colloids and Surfaces. B, Biointerfaces·A T PoortingaH J Busscher
Aug 28, 2003·Nature Materials·Igor L MedintzJ Matthew Mauro
Oct 24, 2003·Biotechnology and Bioengineering·Hongfei JiaPing Wang
Oct 30, 2003·Critical Reviews in Microbiology·Barbara Masschalck, Chris W Michiels
Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Alexey A VertegelJonathan S Dordick
Mar 24, 2005·Colloids and Surfaces. B, Biointerfaces·Olaf ZschörnigKlaus Arnold
Jun 14, 2005·Advanced Drug Delivery Reviews·Gerard D Wright

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Citations

Apr 28, 2011·Applied Microbiology and Biotechnology·Murugan Veerapandian, Kyusik Yun
Jun 15, 2011·ACS Applied Materials & Interfaces·Shuilin WuPaul K Chu
Jul 8, 2010·ACS Chemical Biology·Thomas C ScanlonKarl E Griswold
Nov 21, 2012·Journal of Biomolecular Structure & Dynamics·Chuka OkoliGunaratna Kuttuva Rajarao
Jan 3, 2012·Journal of Nanobiotechnology·Sven C Feifel, Fred Lisdat
Dec 11, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Yang HuChengyou Kan
Jan 25, 2012·Colloids and Surfaces. B, Biointerfaces·Joey N Talbert, Julie M Goddard
Sep 16, 2008·Drug Discovery Today·Nilu JainRoop K Khar
Nov 22, 2014·Nano LIFE·John N Barry, Alexey A Vertegel
Sep 22, 2017·Materials·Oldřich NedělaVáclav Švorčík
Jul 8, 2014·Chemical Communications : Chem Comm·Nirmalya TripathyYoon-Bong Hahn
Dec 31, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Gil YeroslavskyShai Rahimipour
Oct 2, 2021·Bioprocess and Biosystems Engineering·Mozhgan RazzaghiHafiz M N Iqbal

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