Charge Distribution between Different Classes of Glycerophospolipids in MALDI-MS Imaging.

Analytical Chemistry
Marcel S Boskamp, Jens Soltwisch

Abstract

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) is widely used to visualize and analyze the distribution of membrane lipids in an increasingly large number of applications. In this context, different lipoforms of glycerophospholipids (GPLs) are among the prime targets of interest. For this group of analytes, however, ion suppression effects have been described to strongly favor the detection of certain GPL classes over others, thereby hampering the analysis of suppressed species and greatly restraining quantitative analysis. These effects are generally attributed to the distribution of available charge carriers during the MALDI process. Here, we present a systematic investigation of charge distribution between different classes of GPL under MALDI-MSI conditions. For this, we constructed arrays of artificial tissues with different formulated lipid composition that contained predefined amounts of only two specific GPL classes and analyzed them with MALDI-MSI in positive- and negative-ion modes. Next to a characterization of expected ion suppression effects, analysis of these binary systems revealed yet undescribed signal intensity enhancement for the combinations of certain GPL classes. Furthermo...Continue Reading

References

Dec 13, 2000·Journal of Mass Spectrometry : JMS·R KnochenmussR Zenobi
Jun 21, 2003·Clinical Chemistry·Thomas M Annesley
Oct 19, 2006·The Analyst·Richard Knochenmuss
Dec 4, 2008·Journal of Lipid Research·Robert C MurphyRobert M Barkley
Sep 18, 2009·Rapid Communications in Mass Spectrometry : RCM·Yuki Sugiura, Mitsutoshi Setou
May 26, 2011·Cold Spring Harbor Perspectives in Biology·Dominik SchwudkeAndrej Shevchenko
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Thorsten W Jaskolla, Michael Karas
Sep 29, 2011·Rapid Communications in Mass Spectrometry : RCM·Rachelle R LandgrafRichard A Yost
Jun 14, 2012·Chemistry and Physics of Lipids·Louis J SparveroValerian E Kagan
Jul 31, 2012·Journal of Proteomics·Brendan Prideaux, Markus Stoeckli
Dec 26, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Junhwan Kim, Charles L Hoppel
Apr 30, 2013·Progress in Lipid Research·Shane R EllisTodd W Mitchell
Nov 13, 2013·Analytical Chemistry·Min LiHuwei Liu
May 1, 1993·Journal of the American Society for Mass Spectrometry·B H WangF Hillenkamp
Nov 28, 2013·Analytical and Bioanalytical Chemistry·Shane R EllisRon M A Heeren
Jan 8, 2014·Journal of the American Society for Mass Spectrometry·Kuan Yu ChuChi-Kung Ni
Mar 29, 2014·Rapid Communications in Mass Spectrometry : RCM·Laura TomlinsonPeter S Marshall
Jun 21, 2014·Analytical Chemistry·A A AmoscatoV E Kagan
Mar 7, 2015·Science·Jens SoltwischKlaus Dreisewerd
Jul 15, 2015·Annual Review of Analytical Chemistry·Yong Jin Bae, Myung Soo Kim
Jul 26, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Emine B Yalcin, Suzanne M de la Monte
Aug 13, 2015·Journal of the American Society for Mass Spectrometry·Richard Knochenmuss
Aug 19, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jyrki Viidanoja
Oct 1, 2015·Journal of the American Society for Mass Spectrometry·Martin KörsgenHeinrich F Arlinghaus
May 24, 2016·Analytical Chemistry·Simeon Vens-CappellKlaus Dreisewerd
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Marcel WiegelmannJens Soltwisch
Aug 27, 2016·Journal of the American Society for Mass Spectrometry·Milad Nazari, David C Muddiman
Apr 30, 2017·Journal of the American Society for Mass Spectrometry·Mario F Mirabelli, Renato Zenobi
Nov 21, 2017·Analytical Chemistry·Amanda Rae BuchbergerLingjun Li
Jan 4, 2018·Analytical Chemistry·Sebastiaan Van NuffelAlain Brunelle
Feb 21, 2018·Analytical Chemistry·Adam J TaylorJosephine Bunch
May 23, 2018·Nature Methods·Shane R EllisRon M A Heeren
Nov 16, 2018·Rapid Communications in Mass Spectrometry : RCM·Gongyu LiLingjun Li
Aug 9, 2019·Journal of the American Society for Mass Spectrometry·Jonas B MetternichRenato Zenobi

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Citations

Sep 26, 2020·Analytical Chemistry·Bram HeijsKlaus Dreisewerd
Jul 17, 2020·Journal of the American Society for Mass Spectrometry·Alexander PotthoffJens Soltwisch
Sep 19, 2020·Journal of the American Society for Mass Spectrometry·Marcel NiehausJosephine Bunch
Sep 14, 2021·Journal of the American Society for Mass Spectrometry·Josiah C McMillenRichard M Caprioli

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