Charge-Driven Interaction of Antimicrobial Peptide NK-2 with Phospholipid Membranes

ACS Omega
Sanat KarmakarAnimesh Halder

Abstract

NK-2, derived from a cationic core region of NK-lysin, displays antimicrobial activity toward negatively charged bacterial membranes. We have studied the interaction of NK-2 with various phospholipid membranes, using a variety of experimental techniques, such as, isothermal titration calorimetry (ITC), ζ potential, and dynamic light scattering. As bacteria mimicking membranes, we have chosen large unilamellar vesicles (LUVs) composed of negatively charged phospholipid and neutral phospholipids. ITC and ζ potential results show the stronger binding affinity of NK-2 to negatively charged membranes than to neutral membranes. Saturation of the isotherm, obtained from ITC, at a given lipid to NK-2 ratio, was found to be consistent with the charge compensation, determined from ζ potential. A surface partition model with electrostatic contribution was used to estimate the intrinsic binding constant and other thermodynamical parameters of binding kinetics of NK-2. The size distribution of negatively charged LUV in the presence of NK-2 was found to increase drastically, indicating the presence of large aggregates. Such a large aggregate has not been observed in neutral membranes, which supports the ITC and ζ potential results.

References

Oct 1, 1986·Biophysical Journal·H Vogel, F Jähnig
Dec 1, 1993·Protein Science : a Publication of the Protein Society·B BechingerS J Opella
Apr 25, 2000·Medical Microbiology and Immunology·J Andrä, M Leippe
Jan 25, 2002·Nature·Michael Zasloff
Jan 25, 2003·Antimicrobial Agents and Chemotherapy·Thomas JacobsMatthias Leippe
Mar 24, 2004·Biochemistry·Ming-Tao LeeHuey W Huang
Apr 24, 2004·Antimicrobial Agents and Chemotherapy·Jörg AndräKlaus Brandenburg
Jun 1, 2004·Physical Review Letters·Huey W HuangMing-Tao Lee
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
May 17, 2005·Biochimica Et Biophysica Acta·Regine WillumeitJörg Andrä
Nov 13, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Claudia OlakGerald Brezesinski
Jan 1, 2008·Journal of the American Chemical Society·Matthias Meier, Joachim Seelig
Apr 1, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ming-Tao LeeHuey W Huang
Mar 10, 2009·The Journal of Physical Chemistry. B·Yukihiro Tamba, Masahito Yamazaki
Jul 19, 2006·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Rumiana DimovaReinhard Lipowsky
Jun 13, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Tatiana M DominguesKarin A Riske
Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ming-Tao LeeHuey W Huang
Sep 6, 2014·Current Pharmaceutical Design·Nikola StrempelJoerg Overhage
Mar 10, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Mohammad Abu Sayem KaralMasahito Yamazaki
Apr 29, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Corina CiobanasuRegine Willumeit
Jan 15, 2016·Current Biology : CB·Ling-Juan Zhang, Richard L Gallo
Feb 10, 2017·The Journal of Physical Chemistry. B·Achinta SannigrahiKrishnananda Chattopadhyay

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Citations

Jan 9, 2020·BioMed Research International·Germán R Jiménez-GastélumErika L Silva-Benítez
Feb 2, 2021·Chemical Reviews·Jasmin PortelinhaAlfredo M Angeles-Boza
Jan 25, 2020·Biochimie·Sathishkumar MunusamyCarlos Munoz-Garay
Mar 3, 2021·Biochimica Et Biophysica Acta. Biomembranes·Saheli MitraSajal Kumar Ghosh
Mar 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Animesh HalderSanat Karmakar
Dec 4, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Jyotsna Bhatt MitraMukesh Kumar

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Methods Mentioned

BETA
X-ray
isothermal titration calorimetry
fluorescence spectroscopy
isothermal
dynamic light scattering
infrared spectroscopy

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