Charge effects for differentiation of oligodeoxynucleotide isomers containing 8-oxo-dG residues

Journal of the American Society for Mass Spectrometry
Hai LuoRichard D Smith

Abstract

Dissociation reactions of a series of multiply charged oligodeoxynucleotide (ODN) 12-mer anions were studied using an ion trap mass spectrometer. These mixed nucleobase 12-mers fragment first by loss of a neutral nucleobase (A, G, C, and/or 5-methyl-cytosine) followed by cleavage at 3' C-O bond of the sugar from which the base is lost to produce the complementary sequence ions, i.e., [a - B] and w type of ions. No detectable loss of 8-oxo-guanine and/or thymine from these 12-mers is observed under gentle collision conditions in the ion trap. The primary loss of a nucleobase and the subsequent backbone cleavage to generate sequence ions strongly depend on the charge state of the parent molecular ion. For low charge states (2- and 3-), product ions due to the loss of a neutral guanine base and related sequence ions are dominant in the tandem mass spectra. However, preferential loss of a neutral adenine becomes the primary reaction channel from the 5- charge state of the molecular ion. Such charge state dependent fragmentation behavior was utilized to determine the site of 8-oxo-dG residue in a series of structural isomers. The position of 8-oxo-dG residue can be simply determined from the fragmentation pattern of 3- charge state,...Continue Reading

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Citations

Dec 31, 2003·Journal of the American Society for Mass Spectrometry·Rambod Daneshfar, John S Klassen
Mar 30, 2004·Journal of the American Society for Mass Spectrometry·Herbert OberacherChristian G Huber
Jan 18, 2005·Journal of the American Society for Mass Spectrometry·Karin M Keller, Jennifer S Brodbelt
Oct 4, 2005·Journal of the American Society for Mass Spectrometry·Su PanJeehiun K Lee
Oct 22, 2002·Journal of the American Society for Mass Spectrometry·Yinsheng WangShetty Vivekananda
Dec 15, 2010·Analytical Chemistry·Suncerae I Smith, Jennifer S Brodbelt
Jul 19, 2002·Journal of Mass Spectrometry : JMS
Dec 1, 2005·Journal of Mass Spectrometry : JMS·Corinne BuréAgnès F Delmas

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