Charge modifications to improve the emulsifying properties of whey protein isolate

Journal of Agricultural and Food Chemistry
Hairan MaHarry Boer

Abstract

Whey protein isolate was modified by ethylene diamine in order to shift its isoelectric point to an alkaline pH. The extent of the modification was studied using SDS-PAGE and MALDI-TOF mass spectrometry. The modified whey proteins were used as an emulsifier to stabilize oil-in-water emulsions at acidic and neutral pH ranges, and their emulsifying properties were compared with that of the unmodified whey proteins and with the previously studied ethylene diamine modified sodium caseinate. The emulsifying activity of the modified whey proteins was similar to that of the unmodified ones, but the stability of an emulsion at pH 5 was significantly improved after the modification. Charge and coverage of droplet surface and the displacement of the interfacial proteins by surfactant Tween 20 were further studied as a function of pH. As compared with the unmodified whey proteins, the modified ones were proven to cover the interface more efficiently with extensive surface charge at pH 5, although the interfacial layer was less resistant to the surfactant displacement.

References

Nov 24, 1999·Journal of Agricultural and Food Chemistry·M Liu, S Damodaran
Feb 15, 2001·Colloids and Surfaces. B, Biointerfaces·E Dickinson
Apr 19, 2003·Journal of Colloid and Interface Science·Anneke H MartinTon van Vliet
Dec 3, 2003·Advances in Food and Nutrition Research·Jean-Marc Chobert
Apr 10, 2004·Advances in Colloid and Interface Science·Peter WildeVictor Morris
Aug 29, 2006·Journal of Colloid and Interface Science·Tim J Wooster, Mary Ann Augustin
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Sarah S KellerbyEric A Decker
Mar 31, 2009·Journal of Agricultural and Food Chemistry·Hairan MaHarry Boer
Jan 21, 2011·Journal of Agricultural and Food Chemistry·Hairan MaHarry Boer

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Citations

Jun 5, 2012·Journal of Agricultural and Food Chemistry·Hairan MaRiitta Partanen
Feb 28, 2014·Journal of Agricultural and Food Chemistry·Iris J Joye, David J McClements

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