Charge reduction of oligonucleotide anions via gas-phase electron transfer to xenon cations

Rapid Communications in Mass Spectrometry : RCM
James L Stephenson, S A McLuckey

Abstract

Multiply-charged anions derived from electrospray (ES) ionization of the oligonucleotide 5'-d(GTCTTAGCGCTAAGAC)-3' have been subjected to electron transfer reactions with ionized xenon in a quadrupole ion trap and found to undergo minimal fragmentation. This observation stands in contrast with electron transfer to rare gases from anions of smaller oligonucleotides which have been shown to undergo extensive fragmentation. The present results are attributed to longer anion lifetimes for the larger oligonucleotide anions (following highly exothermic electron transfer) which then allow for collisional cooling by the helium bath gas. Reduction to singly charged anions with minimal fragmentation is noted, indicating that xenon cations can be considered as a reagent for simplifying ES mass spectra of moderately sized oligonucleotides. The relative merits of the use of xenon cations in the quadropole ion trap for this purpose is discussed.

Citations

Aug 17, 2001·Rapid Communications in Mass Spectrometry : RCM·C FierensA P De Leenheer
Feb 18, 2010·International Journal of Mass Spectrometry·Suncerae I Smith, Jennifer S Brodbelt
Apr 13, 2013·Analytical Chemistry·Jared B ShawJennifer S Brodbelt
May 24, 2005·Journal of the American Society for Mass Spectrometry·Joshua J CoonJohn E P Syka
Sep 19, 2014·Journal of the American Society for Mass Spectrometry·William D Hoffmann, Glen P Jackson
Jun 9, 1999·Mass Spectrometry Reviews·S A McLuckey, J L Stephenson
Nov 3, 2004·Journal of the American Society for Mass Spectrometry·Estee F NaggarJoseph Zaia
Mar 1, 2005·Journal of the American Society for Mass Spectrometry·Cheng ZhaoStanley Bruckenstein
Jan 8, 1999·Journal of the American Society for Mass Spectrometry·J L Stephenson, S A McLuckey
Dec 18, 2007·Journal of the American Society for Mass Spectrometry·Christopher K BarlowScott A McLuckey
Jun 1, 2017·Journal of the American Society for Mass Spectrometry·Sandilya V B GarimellaRichard D Smith
Feb 25, 2020·Analytical Chemistry·Trenton M Peters-ClarkeJoshua J Coon
Apr 2, 2002·Analytical Chemistry·Scott A McLuckeyGregory B Hurst
Jan 8, 1999·Science·M ScalfL M Smith

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