Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery

Oncotarget
Rong-Qiao CaiSi-Yuan Zhou

Abstract

Bcl-2 gene is an important target to treat lung cancer. The small interference RNA (siRNA) of Bcl-2 gene (siBcl-2) can specifically silence Bcl-2 gene. However, naked siBcl-2 is difficult to accumulate in the tumor tissue to exert its activity. In this paper, a calcium phosphate lipid hybrid nanoparticle that possessed charge reversible property was prepared to enhance the activity of siBcl-2 in vivo. The average diameter and zeta potential of siBcl-2 loaded calcium phosphate lipid hybrid nanoparticles (LNPS@siBcl-2) were 80 nm and -13 mV at pH7.4 whereas the diameter and zeta potential changed to 1506 nm and +9 mV at pH5.0. LNPS@siBcl-2 could efficiently deliver siBcl-2 to the cytoplasm and significantly decreased the expression of Bcl-2 in A549 cells. Moreover, the in vivo experimental results showed that most of the Cy5-siBcl-2 accumulated in tumor tissue after LNPS@Cy5-siBcl-2 was administered to tumor-bearing mice by tail vein injection. Meanwhile, the expression of Bcl-2 was decreased but the expression of the BAX and Caspase-3 was increased in tumor tissue. LNPS@siBcl-2 significantly inhibited the growth of tumor in tumor-bearing mice without any obvious systemic toxicity. Thus, the charge reversible calcium phosphate li...Continue Reading

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Citations

Jan 18, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Wei-Liang YeSi-Yuan Zhou
May 23, 2019·Therapeutic Delivery·Denish BardoliwalaAmbikanandan Misra
Jun 20, 2020·Nanomedicine·Inês MóIlídio J Correia
Dec 10, 2019·International Journal of Pharmaceutics·Zengjun FangXiaoqing Cai
May 11, 2021·Biomedical Materials·Qingmei SunJianzhong Du

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Methods Mentioned

BETA
transfection
transfect
confocal microscopy
flow cytometry
xenograft
dynamic light scattering
transmission electron microscopy
confocal scan microscopy
confocal

Software Mentioned

SPSS
Image Pro Plus

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