Charged amino acids in the AMPV fusion protein have more influence on induced protection than deletion of the SH or G genes

Vaccine
C J NaylorPaul A Brown

Abstract

Modifications to F, G and SH genes of an avian metapneumovirus (AMPV) field isolate were made by reverse genetics and their virulence and protective capacity were tested in young turkeys. Infection of one-day-old turkeys with a subtype A AMPV neither caused disease nor stimulated detectable protection against subsequent virulent challenge. While serial passage of this virus in tracheal tissue increased virulence, protection stimulated remained moderate. Substitution of the fusion protein from a protective AMPV very minimally increasing virulence but dramatically increased induced protection; and this was associated with five amino acid substitutions all involving charged amino acids which computational analysis predicted to affect protein surface properties but not immunodominant helper T-lymphocyte antigenic sites. When SH or G genes were deleted, viruses caused no disease but still conferred full protection to the majority of turkeys. In the case of the SH deletion, shed virus post-inoculation was undetectable. Partial SH deletions were found to confer protection related to the length of SH open reading frame remaining. Removal of both SH and G genes together produced a virus conferring negligible protection. We conclude that...Continue Reading

References

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Nov 26, 2009·Vaccine·Elena CatelliClive J Naylor
Nov 26, 2009·Avian Pathology : Journal of the W.V.P.A·Dennis Rubbenstroth, Silke Rautenschlein

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Citations

Sep 7, 2013·Journal of Medical Virology·A B TedcastleG L Toms
Aug 15, 2020·Transboundary and Emerging Diseases·Giulia MescoliniElena Catelli
Jul 10, 2020·Veterinary Research·Giovanni FranzoMattia Cecchinato

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