Che-1 inhibits oxygen-glucose deprivation/reoxygenation-induced neuronal apoptosis associated with inhibition of the p53-mediated proapoptotic signaling pathway

Neuroreport
Shenglong GuoZhongliang Wu

Abstract

Accumulating evidence suggests that Che-1 is a strong antiapoptotic protein and can protect cells against various insults. However, whether Che-1 is involved in the protection of neurons against cerebral ischemia/reperfusion injury remains unclear. In this study, we aimed to investigate the potential role of Che-1 in regulating cerebral ischemia/reperfusion injury-induced neuronal injury using the oxygen-glucose deprivation and reoxygenation (OGD/R) model in vitro. We found that Che-1 expression was induced in neurons following OGD/R treatment. Functional experiments showed that Che-1 knockdown aggravated OGD/R-induced neuronal apoptosis. In contrast, Che-1 overexpression exerted a protective effect against OGD/R-induced neuronal apoptosis. Moreover, our results showed that the protective effect of Che-1 was associated with the inhibition of p53-mediated proapoptotic genes, including Puma, Noxa, and Bax. In addition, we showed that Che-1 impeded the transcript activity of p53 toward apoptosis. Taken together, our results indicate that Che-1 alleviates OGD/R-induced neuronal apoptosis in-vitro through inhibition of p53-mediated proapoptotic signaling. Our study suggests that Che-1 may serve as a potential therapeutic target for ...Continue Reading

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Citations

May 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi ZhangWei-Juan Gao
Aug 1, 2019·Scientific Reports·Rainer W J KaiserFrancesca Fabretti
Jun 27, 2020·Frontiers in Oncology·Rainer W J KaiserRoman-Ulrich Müller
Nov 5, 2020·Journal of Cellular Physiology·Akshatha N SrinivasDivya P Kumar
Dec 18, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiankun TuSong-Sheng Shi

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Methods Mentioned

BETA
transfection
PCR
Assay
immunoprecipitation
immunoprecipitations
immunoprecipitation assay
ChIP

Software Mentioned

Image
Pro Plus
SPSS

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