PMID: 8524250Dec 1, 1995

Checkpoint genes required to delay cell division in response to nocodazole respond to impaired kinetochore function in the yeast Saccharomyces cerevisiae

Molecular and Cellular Biology
Y Wang, Daniel J Burke


Inhibition of mitosis by antimitotic drugs is thought to occur by destruction of microtubules, causing cells to arrest through the action of one or more mitotic checkpoints. We have patterned experiments in the yeast Saccharomyces cerevisiae after recent studies in mammalian cells that demonstrate the effectiveness of antimitotic drugs at concentrations that maintain spindle structure. We show that low concentrations of nocodazole delay cell division under the control of the previously identified mitotic checkpoint genes BUB1, BUB3, MAD1, and MAD2 and independently of BUB2. The same genes mediate the cell cycle delay induced in ctf13 mutants, limited for an essential kinetochore component. Our data suggest that a low concentration of nocodazole induces a cell cycle delay through checkpoint control that is sensitive to impaired kinetochore function. The BUB2 gene may be part of a separate checkpoint that responds to abnormal spindle structure.


Jan 27, 1997·The Journal of Cell Biology·S S YangK Bloom
Sep 19, 2009·Traffic·Christopher PtakRichard W Wozniak
Jul 22, 2005·Genomics·Silviu L FaitarJohn K Cowell
Apr 30, 2014·Cell Cycle·Yanchang WangKelly McKnight
Sep 2, 1999·Current Biology : CB·S S Taylor
Jan 1, 1996·Annual Review of Genetics·M A Hoyt, J R Geiser
Nov 18, 2003·Annual Review of Genetics·Daniel J Lew, Daniel J Burke
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·R Li
Aug 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aurora A BurdsPeter K Sorger
Mar 21, 2018·Journal of Cell Science·Thibault CourtheouxClaude Prigent
Dec 24, 2004·Molecular Biology of the Cell·Sandra E EncaladaBruce Bowerman
Jan 4, 2008·Molecular Biology of the Cell·Jennifer P BharuchaKelly Tatchell
Sep 11, 2002·Molecular Biology of the Cell·Cheryl D WarrenForrest A Spencer
Oct 21, 2003·Molecular Biology of the Cell·Vladimir S NekrasovJohn V Kilmartin
Mar 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G J Gorbsky
Jul 12, 2018·Molecular Biology of the Cell·Emily L PettyLorraine Pillus
Apr 12, 2007·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, E D Salmon
May 21, 2003·Molecular Genetics and Genomics : MGG·N DeLilloA Vancura
Jun 27, 2003·Molecular Genetics and Genomics : MGG·A A KitazonoS J Kron
Jun 12, 1998·The Journal of Cell Biology·J C WatersE D Salmon
May 5, 1998·Molecular and Cellular Biology·K A Farr, M A Hoyt
Apr 25, 2000·Molecular and Cellular Biology·H LinA Vancura
Jul 13, 2000·Molecular and Cellular Biology·L GlowczewskiM M Smith

Related Concepts

Saccharomyces cerevisiae allergenic extract
Inhibition of Mitosis
BUB3 gene
Cell Division
MAD1L1 gene
BUB1 gene
Essential Hypertension

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.