Chelerythrine induced cell death through ROS-dependent ER stress in human prostate cancer cells

OncoTargets and Therapy
Songjiang WuHaiping Li

Abstract

Prostate cancer is the most common noncutaneous cancer and the second leading cause of cancer-related mortality worldwide and the third in USA in 2017. Chelerythrine (CHE), a naturalbenzo[c]phenanthridine alkaloid, formerly identified as a protein kinase C inhibitor, has also shown anticancer effect through a number of mechanisms. Herein, effect and mechanism of the CHE-induced apoptosis via reactive oxygen species (ROS)-mediated endoplasmic reticulum (ER) stress in prostate cancer cells were studied for the first time. In our present study, we investigated whether CHE induced cell viability decrease, colony formation inhibition, and apoptosis in a dose-dependent manner in PC-3 cells. In addition, we showed that CHE increases intracellular ROS and leads to ROS-dependent ER stress and cell apoptosis. Pre-treatment with N-acetyl cysteine, an ROS scavenger, totally reversed the CHE-induced cancer cell apoptosis as well as ER stress activation, suggesting that the ROS generation was responsible for the anticancer effects of CHE. Taken together, our findings support one of the anticancer mechanisms by which CHE increased ROS accumulation in prostate cancer cells, thereby leading to ER stress and caused intrinsic apoptotic signaling....Continue Reading

Citations

Jul 15, 2020·BMC Cancer·Ana Carolina Martínez-TorresCristina Rodríguez-Padilla
Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Win Sen Heng, Shiau-Chuen Cheah
Oct 17, 2020·BioMed Research International·Chenglin HanXunbo Jin
Aug 29, 2021·Phytochemistry·Toktam AkaberiMaryam Akaberi

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Methods Mentioned

BETA
protein folding
electrophoresis
flow cytometry

Software Mentioned

GraphPad Prism
ImageJ

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