Chelerythrine inhibits Na(+)-H+ exchange in platelets from spontaneously hypertensive rats

Hypertension
O A Gende

Abstract

Hypertension has been associated with increased activity of the Na(+)-H+ exchanger. To study the role played by protein kinase C in this process, we used chelerythrine, a potent and specific inhibitor of the kinase. After an acid load by ammonium chloride preincubation, platelets isolated from spontaneously hypertensive rats showed a faster and larger increase in intracellular pH than platelets from Wistar-Kyoto rats. The initial rate of intracellular pH recovery was 2.46 +/- 0.26 pH units per minute in spontaneously hypertensive rats and 1.74 +/- 0.19 in Wistar-Kyoto rats. For protein kinase C inhibition, platelets were incubated for 30 minutes with 10 mumol/L chelerythrine. This treatment induced a significant reduction in the recovery rate only in spontaneously hypertensive rat platelets, indicating that a pathway involving protein kinase C participates in the prestimulation of the exchanger in cells from this rat strain. Addition of chelerythrine reduced the baseline intracellular pH of platelets. No significant difference was found between the decrease of steady-state intracellular pH induced by chelerythrine in either rat strain. These findings indicate that this model of hypertension is characterized by increased Na(+)-H...Continue Reading

References

May 1, 1992·Clinical Science·G J KempG K Radda
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S WakabayashiJ Pouysségur
May 22, 1990·Biochimica Et Biophysica Acta·F N KoC M Teng
Nov 15, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Jan 1, 1986·The Journal of Membrane Biology·E J Weinman, S Shenolikar
Dec 1, 1988·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·K MurakawaT Takeda
Dec 1, 1988·Journal of Hypertension. Supplement : Official Journal of the International Society of Hypertension·H InaribaT Takeda
Aug 1, 1987·Journal of Molecular and Cellular Cardiology·G J RinaldiH E Cingolani
Dec 15, 1986·Biochemical and Biophysical Research Communications·K TakaoriT Takeda
Jan 1, 1995·The American Journal of Physiology·G HoffmannR Düsing
May 1, 1993·Hypertension·D RosskopfW Siffert

❮ Previous
Next ❯

Citations

Apr 1, 1999·Archives of Physiology and Biochemistry·O A Gende
Mar 29, 2019·Journal of the American Heart Association·Alejandro Martín IbañezVerónica Celeste De Giusti
Aug 5, 2006·The Journal of Experimental Biology·Khaled H AhmedGerhard Krumschnabel

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.