Abstract
Chemerin is a novel adipocyte-derived factor that induces insulin resistance in skeletal muscle. However, the effect of chemerin on skeletal muscle mitochondrial function has received little attention. In the present study, we investigated whether mitochondrial dysfunction is involved in the pathogenesis of chemerin-mediated insulin resistance. In this study, we used recombinant adenovirus to express murine chemerin in C57BL/6 mice. The mitochondrial function and structure were evaluated in isolated soleus muscles from mice. The oxidative mechanism of mitochondrial dysfunction in cultured C2C12 myotubes exposed to recombinant chemerin was analysed by western blotting, immunofluorescence and quantitative real-time polymerase chain reaction. The overexpression of chemerin in mice reduced the muscle mitochondrial content and increased mitochondrial autophagy, as determined by the increased conversion of LC3-I to LC3-II and higher expression levels of Beclin1 and autophagy-related protein-5 and 7. The chemerin treatment of C2C12 myotubes increased the generation of mitochondrial reactive oxygen species, concomitant with a reduced mitochondrial membrane potential and increased the occurrence of mitochondrial protein carbonyls and mi...Continue Reading
References
Oct 8, 2003·The Journal of Experimental Medicine·Valérie WittamerDavid Communi
May 26, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Helene PelicanoPeng Huang
Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Brian A ZabelEugene C Butcher
Mar 1, 2005·Cell·Robert S BalabanToren Finkel
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Li YuMichael Lenardo
Aug 3, 2006·Leukemia Research·Wenbin QianWeilai Xu
Sep 5, 2007·Biochemical and Biophysical Research Communications·Sang-gun RohShin-ichi Sasaki
Jan 19, 2008·Endocrinology·Anthony E Civitarese, Eric Ravussin
Feb 5, 2008·FEBS Letters·Michiko TakahashiKazuo Chihara
Mar 10, 2009·Archives of Physiology and Biochemistry·Antonio ZorzanoManuel Palacín
Sep 2, 2009·Diabetes·Henrike SellJürgen Eckel
Oct 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Souphalone LuangsayMarc Parmentier
Jul 24, 2010·Annals of the New York Academy of Sciences·Hai BoLi Li Ji
Aug 18, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Zoltan UngvariAnna Csiszar
Apr 5, 2011·Human Molecular Genetics·Maria ManczakP Hemachandra Reddy
Jul 2, 2011·Current Pharmaceutical Design·Raquel S CarreiraRoberta A Gottlieb
Aug 9, 2011·The Journal of Nutritional Biochemistry·Jiong YanJiankang Liu
Nov 16, 2011·Molecular and Cellular Biology·Huei-Fen JhengYau-Sheng Tsai
Mar 8, 2012·American Journal of Physiology. Endocrinology and Metabolism·Scott K PowersHaydar A Demirel
Apr 18, 2012·PloS One·Sang Hui ChuJi Won Lee
Citations
Jun 10, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ou ZhangYu-Jie Zhou
May 18, 2016·Free Radical Biology & Medicine·George G RodneyReem Abo-Zahrah
Oct 6, 2016·Ageing Research Reviews·Alexander Kalinkovich, Gregory Livshits
Nov 12, 2016·Scientific Reports·Jing YuAyong Zhao
Dec 19, 2017·Biomedit︠s︡inskai︠a︡ khimii︠a︡·M A VasilenkoL S Litvinova
Aug 20, 2016·Nature Communications·Ralph KloseChristian Stockmann
Jul 26, 2019·Connective Tissue Research·Jianheng ZhengMengwei Sun
Jul 15, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrea ArmaniMassimiliano Caprio
Oct 24, 2019·Animals : an Open Access Journal From MDPI·Bianhong HuDebing Yu
Feb 13, 2018·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·D A SkuratovskaiaI O Mazunin
Jun 19, 2019·Journal of Lipid Research·Jan-Bernd Funcke, Philipp E Scherer
Mar 16, 2019·BMC Medical Genomics·Daria SkuratovskaiaLarisa Litvinova
Sep 10, 2020·PeerJ·Daria SkuratovskaiaLarisa Litvinova
Aug 28, 2021·International Journal of Molecular Sciences·Nicholas A ForemanLi Li Ji