Chemerin-induced mitochondrial dysfunction in skeletal muscle

Journal of Cellular and Molecular Medicine
Qihai XiePingjin Gao

Abstract

Chemerin is a novel adipocyte-derived factor that induces insulin resistance in skeletal muscle. However, the effect of chemerin on skeletal muscle mitochondrial function has received little attention. In the present study, we investigated whether mitochondrial dysfunction is involved in the pathogenesis of chemerin-mediated insulin resistance. In this study, we used recombinant adenovirus to express murine chemerin in C57BL/6 mice. The mitochondrial function and structure were evaluated in isolated soleus muscles from mice. The oxidative mechanism of mitochondrial dysfunction in cultured C2C12 myotubes exposed to recombinant chemerin was analysed by western blotting, immunofluorescence and quantitative real-time polymerase chain reaction. The overexpression of chemerin in mice reduced the muscle mitochondrial content and increased mitochondrial autophagy, as determined by the increased conversion of LC3-I to LC3-II and higher expression levels of Beclin1 and autophagy-related protein-5 and 7. The chemerin treatment of C2C12 myotubes increased the generation of mitochondrial reactive oxygen species, concomitant with a reduced mitochondrial membrane potential and increased the occurrence of mitochondrial protein carbonyls and mi...Continue Reading

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Citations

Jun 10, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ou ZhangYu-Jie Zhou
May 18, 2016·Free Radical Biology & Medicine·George G RodneyReem Abo-Zahrah
Dec 19, 2017·Biomedit︠s︡inskai︠a︡ khimii︠a︡·M A VasilenkoL S Litvinova
Jul 26, 2019·Connective Tissue Research·Jianheng ZhengMengwei Sun
Jul 15, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrea ArmaniMassimiliano Caprio
Oct 24, 2019·Animals : an Open Access Journal From MDPI·Bianhong HuDebing Yu
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Jun 19, 2019·Journal of Lipid Research·Jan-Bernd Funcke, Philipp E Scherer
Aug 28, 2021·International Journal of Molecular Sciences·Nicholas A ForemanLi Li Ji

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Methods Mentioned

BETA
PCR
transfection

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