Chemical anchoring of lauryl methacrylate-based reversed phase monolith to 1/16″ o.d. polyetheretherketone tubing

Journal of Chromatography. a
Shin ShuTomonari Umemura

Abstract

In this paper, we describe a method for the preparation of easy-to-use reversed-phase monolithic microbore columns. Polyetheretherketone (PEEK) tubing with an outer diameter of 1/16″ and an inner diameter of 1.0 mm was used as a column housing (empty column), and in it lauryl methacrylate (LMA) was copolymerized with ethylene dimethacrylate (EDMA). In order to chemically anchor the polymer monolith to the tube wall, the inner wall surface was pretreated by the following two-step procedure. (1) 50% sulfuric acid was filled into the PEEK tubing and left to stand for 6 h to generate sulfonate groups on the surface. (2) After washing with Milli-Q water, the sulfonated PEEK surface was brought into contact with 1 M glycidyl methacrylate in dichloromethane (or acetone) at 40°C for 4 h to introduce methacryloyl groups via the reaction of sulfonate groups and epoxy groups. Mechanical strength and column efficiency of the resulting monoliths were evaluated through the separation of a series of alkylbenzenes in acetonitrile-water (50:50, v/v) eluent over the flow rate range of 50-750 μL/min (corresponding to 1.7-25.5 mm/s). The poly(LMA-co-EDMA) monolith provided acceptable column efficiency of 2000 theoretical plates/10 cm (HETP value o...Continue Reading

References

Mar 5, 2002·Journal of Chromatography. a·Pavel CoufalKarel Stulík
Nov 29, 2002·Journal of Chromatography. a·Majed Al-BokariGeorges Guiochon
May 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Karel StulíkPavel Coufal
Oct 5, 2010·Journal of Chromatography. a·Joseph ChamiehAgnès Hagège

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Citations

Jun 28, 2014·The Journal of Prosthetic Dentistry·Bogna StawarczykChristine Keul
Nov 23, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoyan JiaoTiesheng Shi
Jul 3, 2013·Journal of Separation Science·Kun LiuMilton L Lee
Sep 12, 2020·Journal of Separation Science·Fernando Henrique do NascimentoJorge Cesar Masini

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