Jun 1, 1977

Chemical and immunogenic properties of the capsular antigen of Staphylococcus aureus. I. purification and chemical characteristics of the capsular antigen

Zhurnal mikrobiologii, epidemiologii, i immunobiologii
G SeltmannV Ia Prokhorov

Abstract

Crude capsular material isolated from S. aureus strain 1193/74 was characterized chemically and subjected to purification by 4 different methods. The best results were obtained by the method including deproteinization of the material with a mixture of chloroform and butanol, fractional precipitation with ethanol and separation on SE-Sephadex. Galactose, uronic acids, and a small amount of protein served as components characteristic of the purified capsular material. Galacturonic and glucuronic acids were identified amoung the uronic acids. Besides, there were revealed other acids with a carbohydrate base, whose nature is still obscure.

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Mentioned in this Paper

Ethanol
Butanols
Galactose Measurement
Ethanol Measurement
Thylacodes aureus
Chromatography, DEAE-Cellulose
1-Butanol
Fractional Precipitation
Glucuronic Acids
Galactose

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