Chemical and pharmacological investigation of micropropagated Hygrophila pogonocalyx produced from leaf explants

Botanical Studies
Chin-Wen HoMei-Hsien Lee

Abstract

An optimized method for indirect shoot organogenesis from the leaf explants of Hygrophila pogonocalyx, a rare and endemic species in Taiwan, was developed to supply enough quantity of plant materials for the first chemical and pharmacological investigation. Incubation of the young leaves on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (0.5 mg/l) and indole-3-acetic acid (0.1 mg/l) resulted in the best multiplication rate for organogenesis. The average number of adventitious buds per leaf was 22.8 ± 1.9 after 8-week culture. The adventitious buds rooted and developed into plantlets when cultured simply on MS medium. Using this protocol, up to 37,600 plants were produced from a single leaf explant in one year. From the ethanol extract of the leaves of this micropropagated plant, 13 compounds were isolated and identified, including two flavones (1, 11), four flavonols (9, 10, 12, and 13), three phenylethanoid glycosides (6-8), two alkylated glycosides (2-3), and two steroids (4-5). Of these, acteoside (7) exhibited anti-tyrosinase activity in human epidermal melanocytes and luteolin 7-O-β-D-glucopyranoside (11) exhibited the greatest neurocytoprotective activity. The method, indirect shoot organogenesis fr...Continue Reading

References

Sep 6, 2001·Archives of Pharmacal Research·H J KimY S Lee
Dec 12, 2001·Phytochemistry·I I MahmoudA M Hassan
Jun 6, 2002·Fitoterapia·M R Khan, A D Omoloso
Jan 4, 2003·Archives of Pharmacal Research·Ming Hong LeeYong Nam Han
May 3, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·W C HouM H Lee
May 29, 2003·Phytochemistry·Jun WuLiangmin Huang
Jul 3, 2004·The Journal of Pharmacy and Pharmacology·Wen Fei ChiouChieh Fu Chen
Oct 11, 2005·Journal of Ethnopharmacology·P Shanmugasundaram, S Venkataraman
Nov 18, 2005·Journal of Ethnopharmacology·M VijayakumarP Pushpangadan
Jul 19, 2008·Journal of Asian Natural Products Research·Jian ZouWei-Min Zhao
Apr 24, 2009·Neurochemical Research·Sebastian P FernandezMary Chebib
Jul 25, 2009·Phytochemistry·Yi-Pei LinShui-Tein Chen
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guo-An ZouZhong-Mei Zou
Nov 6, 2010·Archives of Pharmacal Research·Byung Sun MinTran Manh Hung
Nov 20, 2010·Molecular Nutrition & Food Research·Yong Pil HwangHye Gwang Jeong
Feb 18, 2011·Natural Product Research·Shunsuke ShimosakiMorihiko Nakamura
May 3, 2011·Journal of Asian Natural Products Research·Jiang HeZhen-Dan He
Jun 22, 2011·Progress in Neuro-psychopharmacology & Biological Psychiatry·M PereiraR Andreatini
Sep 9, 2011·The Journal of Pharmacy and Pharmacology·Young-Ok SonJeong-Chae Lee
Mar 9, 2012·Advances in Experimental Medicine and Biology·Hsin-Chen Lee, Yau-Huei Wei

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Methods Mentioned

BETA
protein assay
enzyme-linked immunosorbent assay
column chromatography

Software Mentioned

Statistical Analysis system ( SAS )

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