PMID: 1236742Aug 26, 1975Paper

Chemical and physical properties of the disulfides of bovine neurophysin-II

Biochemistry
C J Menendez-Botet, E Breslow

Abstract

Bovine neurophysin-II is shown to be very susceptible to partial reduction in the absence of urea. Reduction of an average of one disulfide leads to major changes in conformation and disulfide optical activity, manifest in part by pronounced far-uv ellipticity changes, complete loss of the 248-nm ellipticity band, and a shift of the 278-nm ellipticity band to shorter wavelengths with loss of half its intensity; the reduction process generates a mixture of products and appears to be accompanied by disulfide interchange. The circular dichroism data indicate that the disulfide(s) most susceptible to reduction or interchange are either the principal contributors to the 248- and 278-nm ellipticity bands or that the optical activity of other disulfides is dependent on their integrity. Peptides that bind to the hormone-binding site of neurophysin-II protect against reduction. On reoxidation of partially reduced neurophysin-II there is only a partial return of the native circular dichroism spectrum and electrophoretic behavior. The percentage of native protein in samples reoxidized following different degrees of reduction was estimated by comparison of the circular dichroism spectra of these samples with those of the fractionated nativ...Continue Reading

Citations

Nov 1, 1979·Angewandte Chemie·R Acher
Feb 1, 1996·Nature Structural Biology·J P RoseB C Wang
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S BurmanE Breslow
Nov 15, 1976·FEBS Letters·M ChauvetR Acher
Jun 8, 2001·The Journal of Biological Chemistry·S EubanksE Breslow
Nov 1, 1978·International Journal of Peptide and Protein Research·T A Bewley, Y Birk
Mar 1, 1980·International Journal of Peptide and Protein Research·Y BirkT A Bewley

Related Concepts

Bos indicus
Circular Dichroism, Vibrational
Disulfides
Sputolysin
SDS-PAGE
Iodoacetamide
Iodoacetic Acids
Neurophysins
Oxidation-Reduction
Plasma Protein Binding Capacity

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