Chemical and spatial analysis of protein loaded PLGA microspheres for drug delivery applications

Journal of Controlled Release : Official Journal of the Controlled Release Society
A RafatiMartyn C Davies

Abstract

Polymer microspheres for controlled release of therapeutic protein from within an implantable scaffold were produced and analysed using complimentary techniques to probe the surface and bulk chemistry of the microspheres. Time of Flight - Secondary Ion Mass Spectrometry (ToF-SIMS) surface analysis revealed a thin discontinuous film of polyvinyl alcohol (PVA) surfactant (circa 4.5 nm thick) at the surface which was readily removed under sputtering with C(60). Atomic Force Microscopy (AFM) imaging of microspheres before and after sputtering confirmed that the PVA layer was removed after sputtering revealing poly(lactic-co-glycolic) acid(PLGA). Scanning electron microscopy showed the spheres to be smooth with some shallow and generally circular depressions, often with pores in their central region. The occurrence of the protein at the surface was limited to areas surrounding these surface pores. This surface protein distribution is believed to be related to a burst release of the protein on dissolution. Analysis of the bulk properties of the microspheres by confocal Raman mapping revealed the 3D distribution of the protein showing large voids within the pores. Protein was found to be adsorbed at the interface with the PLGA oil pha...Continue Reading

References

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Citations

Apr 5, 2014·Colloids and Surfaces. B, Biointerfaces·Tongtong MaSiling Wang
Jan 31, 2015·Advanced Drug Delivery Reviews·Geoffrey P S SmithKeith C Gordon
Mar 1, 2016·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·N VargaI Dékány
Oct 25, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Huijie Zhang, Xiao-Dong Gao
May 29, 2013·Colloids and Surfaces. B, Biointerfaces·Michał Cieśla, Jakub Barbasz
Feb 9, 2018·Journal of Drug Targeting·Derfogail DelcassianRobert Langer
Apr 3, 2019·Journal of Biomaterials Science. Polymer Edition·Chengcheng ZhuLian Cen
Aug 5, 2017·Journal of Biomaterials Science. Polymer Edition·Yamina BoukariNashiru Billa
Sep 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Martin R L PaineShane R Ellis
Jan 13, 2018·Biointerphases·Lin DuHeng-Yong Nie
Feb 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sayeed HasanClive A Prestidge
Oct 14, 2019·Journal of Pharmaceutical Sciences·Khanh T T NguyenWouter L J Hinrichs

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