Chemical anoxia activates ATP-sensitive and blocks Ca(2+)-dependent K(+) channels in rat dorsal vagal neurons in situ

Neuroscience
A KulikKlaus Ballanyi

Abstract

The contribution of subclasses of K(+) channels to the response of mammalian neurons to anoxia is not yet clear. We investigated the role of ATP-sensitive (K(ATP)) and Ca(2+)-activated K(+) currents (small conductance, SK, big conductance, BK) in mediating the effects of chemical anoxia by cyanide, as determined by electrophysiological analysis and fluorometric Ca(2+) measurements in dorsal vagal neurons of rat brainstem slices. The cyanide-evoked persistent outward current was abolished by the K(ATP) channel blocker tolbutamide, but not changed by the SK and BK channel blockers apamin or tetraethylammonium. The K(+) channel blockers also revealed that ongoing activation of K(ATP) and SK channels counteracts a tonic, spike-related rise in intracellular Ca(2+) ([Ca(2+)](i)) under normoxic conditions, but did not modify the rise of [Ca(2+)](i) associated with the cyanide-induced outward current. Cyanide depressed the SK channel-mediated afterhyperpolarizing current without changing the depolarization-induced [Ca(2+)](i) transient, but did not affect spike duration that is determined by BK channels. The afterhyperpolarizing current and the concomitant [Ca(2+)](i) rise were abolished by Ca(2+)-free superfusate that changed neither ...Continue Reading

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Citations

Dec 29, 2009·Progress in Neurobiology·Dongman Chao, Ying Xia
Jan 3, 2006·Molecular and Cellular Endocrinology·D MenegazF R M B Silva
Dec 9, 2003·The Journal of Physiology·Friederike Bergmann, Bernhard U Keller
Nov 19, 2013·Neurochemistry International·Jun-Feng WangHong-Gang Pang
Oct 14, 2016·Annals of the New York Academy of Sciences·Calum A MacRaeRandall T Peterson
May 10, 2018·The Journal of Physiology·Jan Marino RamirezIbis M Agosto-Marlin
Aug 10, 2004·The Journal of Experimental Biology·Klaus Ballanyi

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