Chemical, biological, and immunochemical properties of the Chlamydia psittaci lipopolysaccharide.

Infection and Immunity
L BradeH Brade

Abstract

The lipopolysaccharide (LPS) of Chlamydia psittaci was extracted from yolk sac-grown elementary bodies, purified, and characterized chemically, immunochemically, and biologically. The LPS contained D-galactosamine, D-glucosamine, phosphorus, long-chain fatty acids, and 3-deoxy-D-manno-2-octulosonic acid in the molar ratio of approximately 1:2:2:6:5. The antigenic properties of the isolated LPS were compared with those of the LPS from Chlamydia trachomatis and Salmonella minnesota Re by the passive hemolysis and passive hemolysis inhibition tests, absorption, hydrolysis kinetics, and Western blot analysis with rabbit polyclonal antisera against chlamydiae and with a mouse monoclonal antibody recognizing a genus-specific epitope of chlamydial LPS. Two antigenic determinants were identified, one of which was chlamydia specific and the other of which was cross-reactive with Re LPS. Both determinants were destroyed during acid hydrolysis, whereby a third antigen specificity was exposed which was indistinguishable from the lipid A antigenicity. In rabbit polyclonal antisera prepared against Formalin-killed elementary bodies or detergent-solubilized membranes, two antibody specificities were differentiated. One of these was chlamydia ...Continue Reading

References

Dec 1, 1979·Journal of Clinical Microbiology·H Brade, H Brunner
Jun 1, 1975·European Journal of Biochemistry·C Galanos, O Lüderitz
Apr 1, 1985·European Journal of Biochemistry·C GalanosH Yoshimura
May 1, 1985·Infection and Immunity·L BradeH Brade
May 1, 1985·Infection and Immunity·M NurminenH Brade
Dec 1, 1971·Infection and Immunity·S P DhirJ T Grayston
Jun 30, 1966·Annals of the New York Academy of Sciences·O LüderitzJ Schlosshardt
Oct 1, 1968·Journal of Bacteriology·D T Kingsbury, E Weiss
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1980·Annual Review of Microbiology·J Schachter, H D Caldwell
Mar 1, 1982·Infection and Immunity·H D Caldwell, J Schachter

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Citations

Apr 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·L W WoodsA E Castro
Sep 6, 2000·European Journal of Biochemistry·S RundO Holst
Jun 8, 1999·The Journal of Biological Chemistry·S RundO Holst
Mar 29, 2003·The Journal of Peptide Research : Official Journal of the American Peptide Society·C Chong-CerrilloL M de la Maza
Oct 7, 2009·Expert Review of Vaccines·Daniel D RockeyGuangming Zhong
Sep 9, 2020·FEMS Microbiology Reviews·Barbara S Sixt
Oct 14, 2017·The Journal of Infectious Diseases·Patrik M BavoilJacques Ravel
Dec 1, 1996·FEMS Immunology and Medical Microbiology·O HolstE T Rietschel
Sep 1, 1996·Progress in Lipid Research·S G Wilkinson
Nov 26, 1999·Biochimica Et Biophysica Acta·P Kosma
Apr 1, 1989·Clinical Microbiology Reviews·R C Barnes
Jun 1, 1994·Infection and Immunity·M LukácováH Brade
Oct 1, 1992·Infection and Immunity·M BaumannH Brade
Apr 1, 1997·Applied and Environmental Microbiology·A EssigR Marre
Mar 1, 1991·Microbiological Reviews·J W Moulder

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