Chemical, biological and immunological characterization of separated electrophoretic components of human chorionic somatomammotropin (HCS)

International Journal of Peptide and Protein Research
P TarliP Neri

Abstract

Purified HCS preparations ofter show heterogeneity when assayed by electrophoresis, although they may be considered homogeneous according to other criteria (N-terminal amino acid analysis, gel filtration and ultracentrifugation patterns, etc.). Using DEAE-cellulose chromatography, the two main components corresponding to various degrees of hormone amidation were separated. The deamidation kinetic of the electrophoretically slower migrating component was studied and found to be sensitive to temperature and, to a lesser extent, to pH. Denaturing agents strongly increased the deamidation rate, while no effect was found by raising the ionic strength. No difference was evident between the two components and the parent hormone when compared (on a weight basis) in a specific HCS radioimmunoassay system. Even the precipitin reaction in agar gel with an anti-human growth hormone (HGH) serum did not show variations in cross-reactivity versus HGH. Moreover, groups of guinea pigs immunized with the single HCS components, gave antibodies reacting identically with the components and the parent HCS. The prolactin-like activity of the two components differed when determined by the pigeon crop-sac asay: the slower migrating component proved to ...Continue Reading

References

Apr 2, 1974·Archives of Biochemistry and Biophysics·P Tarli, C H Li
Dec 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·L M Sherwood
Oct 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·C ArezziniP Neri
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·A B RobinsonP Cary
Sep 29, 1970·Biochimica Et Biophysica Acta·U J LewisW C Hopkins
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Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jan 1, 1948·Acta Pathologica Et Microbiologica Scandinavica·O OUCHTERLONY

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Citations

Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·H T Wright

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